{"id":"eb98173b-3d91-55c4-b19d-df1615e5e2d6","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-depletion-repletion-ttp-localization","predicate":"changes_localization","statement":"Adding alpha-tocopherol to vitamin E-depleted rat hepatoma cells expressing human TTP changed its punctate perinuclear distribution to a diffuse cellular pattern.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d886ec6b-cb9f-514f-be96-9dadd122484e","mechanism_event_label":"Vitamin E supply changed where its transfer protein accumulated in these cells.","subject":{"id":"ae6048a7-d112-5d3a-8e7c-ad5a152642f9","slug":"rrr-alpha-tocopherol","display_name":"RRR-alpha-tocopherol","entity_type_key":"small_molecule"},"object":{"id":"69f24038-474c-5f6f-ab2b-e3472129e652","slug":"alpha-ttp-intracellular-redistribution","display_name":"Alpha-TTP intracellular redistribution","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d886ec6b-cb9f-514f-be96-9dadd122484e","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-depletion-repletion-ttp-localization-event","event_type":"biochemical_relationship","label":"Vitamin E supply changed where its transfer protein accumulated in these cells.","description":"Adding alpha-tocopherol to vitamin E-depleted rat hepatoma cells expressing human TTP changed its punctate perinuclear distribution to a diffuse cellular pattern.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ae6048a7-d112-5d3a-8e7c-ad5a152642f9","slug":"rrr-alpha-tocopherol","display_name":"RRR-alpha-tocopherol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"69f24038-474c-5f6f-ab2b-e3472129e652","slug":"alpha-ttp-intracellular-redistribution","display_name":"Alpha-TTP intracellular redistribution","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8793fb03-2ab4-5f16-bdbe-c18ca395d7d7","slug":"ttpa","display_name":"Human alpha-tocopherol transfer protein","entity_type_key":"protein"},"role":"expressed_human_protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human TTP expressed in depleted McARH7777 cells; Figure 2A","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"More than 10 passages in alpha-tocopherol-free defined serum; 35 µM serum-complexed d-alpha-tocopherol for 24 hours.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Depletion/repletion model with overexpressed human protein; localization is not a clinical deficiency threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Rattus norvegicus cells; Homo sapiens protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Vitamin E supply changed where its transfer protein accumulated in these cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chung2016] Vitamin E and Phosphoinositides Regulate the Intracellular Localization of the Hepatic α-Tocopherol Transfer Protein. (2016). https://pubmed.ncbi.nlm.nih.gov/27307040/ DOI: 10.1074/jbc.m116.734210","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Hepatoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"585cb910-6150-5912-b782-12d1cb866fa2","evidence_kind":"source_excerpt","locator":"Lines 337-348","start_line":337,"end_line":348,"excerpt":"### ve-transport-depletion-repletion-ttp-localization\nAdding alpha-tocopherol to vitamin E-depleted rat hepatoma cells expressing human TTP changed its punctate perinuclear distribution to a diffuse cellular pattern.\nCondition category: nutrient_deficiency\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Vitamin E supply changed where its transfer protein accumulated in these cells.\norganism: Rattus norvegicus cells; Homo sapiens protein\ntissue_or_cell_type: Hepatoma cells\nexperimental_model: Human TTP expressed in depleted McARH7777 cells; Figure 2A\nlimitations: Depletion/repletion model with overexpressed human protein; localization is not a clinical deficiency threshold.\nexposure: More than 10 passages in alpha-tocopherol-free defined serum; 35 µM serum-complexed d-alpha-tocopherol for 24 hours.\ncross_nutrient: false\n[chung2016] Vitamin E and Phosphoinositides Regulate the Intracellular Localization of the Hepatic α-Tocopherol Transfer Protein. 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