{"id":"eb460d97-0959-59f7-a1de-32751a96eba6","stable_key":"cfa4bd86-d88d-555a-975c-68877bf00e0f:lutein-abca1-trafficking","predicate":"reduces","statement":"The analogous mouse ABCA1 E89K substitution impaired intracellular trafficking, leaving little transporter at the cell surface.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"25f743d8-27a8-5f53-965f-3596266665f0","mechanism_event_label":"The protein failed to reach its working location.","subject":{"id":"fee158cd-4a11-5728-b8b8-07898b679774","slug":"mouse-abca1-e89k","display_name":"Mouse ABCA1 E89K experimental variant","entity_type_key":"protein_state"},"object":{"id":"002a1163-fb8a-5cd8-af33-59d6de488216","slug":"mouse-abca1-surface-localization","display_name":"Mouse ABCA1 cell-surface localization","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"25f743d8-27a8-5f53-965f-3596266665f0","stable_key":"cfa4bd86-d88d-555a-975c-68877bf00e0f:lutein-abca1-trafficking-event","event_type":"biochemical_relationship","label":"The protein failed to reach its working location.","description":"The analogous mouse ABCA1 E89K substitution impaired intracellular trafficking, leaving little transporter at the cell surface.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fee158cd-4a11-5728-b8b8-07898b679774","slug":"mouse-abca1-e89k","display_name":"Mouse ABCA1 E89K experimental variant","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"002a1163-fb8a-5cd8-af33-59d6de488216","slug":"mouse-abca1-surface-localization","display_name":"Mouse ABCA1 cell-surface localization","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/lutein-research/12364545.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e3deaf99143042e2667f6be249df15574ba1f975f3b537f5b779fbe69bd20c4c\", \"start_char\": 0, \"end_char\": 913, \"text_sha256\": \"e3deaf99143042e2667f6be249df15574ba1f975f3b537f5b779fbe69bd20c4c\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"WHAM sequencing and functional expression of corresponding mutation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Naturally occurring chicken E89K; analogous mouse protein substitution","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Trafficking experiment used mouse protein; do not silently relabel it as human or chicken protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lutein research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lutein","display_name":"Lutein","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Chicken genotype and engineered mouse ABCA1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The protein failed to reach its working location.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[lutein-p12364545] Identification and functional analysis of a naturally occurring E89K mutation in the ABCA1 gene of the WHAM chicken. (2002). https://pubmed.ncbi.nlm.nih.gov/12364545/ DOI: 10.1194/jlr.m200223-jlr200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Lipoprotein phenotype and cellular membrane trafficking","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"15cf699b-9669-5e1e-babb-f13e9eba2c31","evidence_kind":"source_excerpt","locator":"Lines 372-383","start_line":372,"end_line":383,"excerpt":"### lutein-abca1-trafficking\nThe analogous mouse ABCA1 E89K substitution impaired intracellular trafficking, leaving little transporter at the cell surface.\nCondition category: machinery_impairment\nnutrient_topic: Lutein research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The protein failed to reach its working location.\norganism: Chicken genotype and engineered mouse ABCA1\ntissue_or_cell_type: Lipoprotein phenotype and cellular membrane trafficking\nexperimental_model: WHAM sequencing and functional expression of corresponding mutation\nlimitations: Trafficking experiment used mouse protein; do not silently relabel it as human or chicken protein.\nexposure: Naturally occurring chicken E89K; analogous mouse protein substitution\nevidence_span: {\"source_cache\": \"artifacts/lutein-research/12364545.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"e3deaf99143042e2667f6be249df15574ba1f975f3b537f5b779fbe69bd20c4c\", \"start_char\": 0, \"end_char\": 913, \"text_sha256\": \"e3deaf99143042e2667f6be249df15574ba1f975f3b537f5b779fbe69bd20c4c\"}\n[lutein-p12364545] Identification and functional analysis of a naturally occurring E89K mutation in the ABCA1 gene of the WHAM chicken. (2002). https://pubmed.ncbi.nlm.nih.gov/12364545/ DOI: 10.1194/jlr.m200223-jlr200","model_system":"WHAM sequencing and functional expression of corresponding mutation","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [lutein-p12364545] Identification and functional analysis of a naturally occurring E89K mutation in the ABCA1 gene of the WHAM chicken. (2002). https://pubmed.ncbi.nlm.nih.gov/12364545/ DOI: 10.1194/jlr.m200223-jlr200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"beef4241-6ebd-5b36-864c-6ef3ed7e61b8","stable_key":"import-cfa4bd86-d88d-555a-975c-68877bf00e0f","title":"Lutein: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"1514ec459913a7044b4c4e1012ed367bcb918298181ebabd2a9e2ccecd789053","revision_id":"bae2c856-0785-546f-b08f-869318c27f9e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}