{"id":"eb43be96-af45-5940-a53f-2c0d12c1bebf","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:mthfs-atp-magnesium-dependence","predicate":"supports","statement":"The purified human liver MTHFS reaction requires ATP and Mg2+ and is an ADP-forming cyclo-ligase reaction.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"e75ec225-4d3f-5443-8801-b0239898ca2b","mechanism_event_label":"ATP supplies energy for this magnesium-dependent folate conversion.","subject":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"object":{"id":"9a1f2454-da04-5158-914f-e3d4d26c61b6","slug":"mthfs","display_name":"Human methenyltetrahydrofolate synthetase / MTHFS","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"e75ec225-4d3f-5443-8801-b0239898ca2b","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:mthfs-atp-magnesium-dependence-event","event_type":"biochemical_relationship","label":"ATP supplies energy for this magnesium-dependent folate conversion.","description":"The purified human liver MTHFS reaction requires ATP and Mg2+ and is an ADP-forming cyclo-ligase reaction.","status":"provisional","compartment":{"slug":"cytosol","display_name":"Cytosol"},"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"required cofactor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"136c3764-1b93-5f79-8673-9001cab9bc3d","slug":"adp","display_name":"Adenosine diphosphate","entity_type_key":"small_molecule"},"role":"nucleotide product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bab4af27-0401-545c-aa8f-e945920e614b","slug":"5-formyltetrahydrofolate","display_name":"5-Formyltetrahydrofolate","entity_type_key":"small_molecule"},"role":"folate substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"9a1f2454-da04-5158-914f-e3d4d26c61b6","slug":"mthfs","display_name":"Human methenyltetrahydrofolate synthetase / MTHFS","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Magnesium and ATP support folate interconversion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified liver enzyme","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Controlled enzyme assay with ATP and magnesium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cofactor dependence does not establish a dietary magnesium threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"ATP supplies energy for this magnesium-dependent folate conversion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[bertrand-1987] Human liver methenyltetrahydrofolate synthetase: improved purification and increased affinity for folate polyglutamate substrates (1987). https://pubmed.ncbi.nlm.nih.gov/3801490/ DOI: 10.1016/0167-4838(87)90004-5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver-derived, cell-free","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bb40606c-5491-53dd-bd70-884f2c002135","evidence_kind":"source_excerpt","locator":"Lines 1255-1266","start_line":1255,"end_line":1266,"excerpt":"### mthfs-atp-magnesium-dependence\nThe purified human liver MTHFS reaction requires ATP and Mg2+ and is an ADP-forming cyclo-ligase reaction.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: ATP supplies energy for this magnesium-dependent folate conversion.\norganism: Homo sapiens\ntissue_or_cell_type: Liver-derived, cell-free\nexperimental_model: Purified liver enzyme\nlimitations: Cofactor dependence does not establish a dietary magnesium threshold.\nexposure: Controlled enzyme assay with ATP and magnesium.\ncross_nutrient: Magnesium and ATP support folate interconversion.\n[bertrand-1987] Human liver methenyltetrahydrofolate synthetase: improved purification and increased affinity for folate polyglutamate substrates (1987). https://pubmed.ncbi.nlm.nih.gov/3801490/ DOI: 10.1016/0167-4838(87)90004-5","model_system":"Purified liver enzyme","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [bertrand-1987] Human liver methenyltetrahydrofolate synthetase: improved purification and increased affinity for folate polyglutamate substrates (1987). https://pubmed.ncbi.nlm.nih.gov/3801490/ DOI: 10.1016/0167-4838(87)90004-5","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}