{"id":"eb038ff8-d577-5c64-80ee-4f4284381061","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-mitochondrial-carrier","predicate":"transports_tested","statement":"Slc25a44 loss reduced mitochondrial valine and leucine uptake; ectopic expression and reconstituted liposomes supported transporter function.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"4422323e-812f-579d-9f19-621c6e3023d6","mechanism_event_label":"Access to mitochondria is a separate gate from entry into the cell.","subject":{"id":"1c24397f-cf67-53b7-810a-17d9f01c6236","slug":"mouse-slc25a44","display_name":"Mouse mitochondrial BCAA carrier / Slc25a44","entity_type_key":"protein"},"object":{"id":"56939e51-c40e-5395-8102-beb5de988d67","slug":"mouse-mitochondrial-bcaa-uptake","display_name":"Mitochondrial BCAA uptake in mouse cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4422323e-812f-579d-9f19-621c6e3023d6","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-mitochondrial-carrier-event","event_type":"observed_relationship","label":"Access to mitochondria is a separate gate from entry into the cell.","description":"Slc25a44 loss reduced mitochondrial valine and leucine uptake; 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ectopic expression and reconstituted liposomes supported transporter function.\nModel: Mouse brown adipocytes/Neuro2a and membrane/proteoliposome assays.\nLimitations: Direct transport tracers highlighted here were valine and leucine; do not relabel them as an isolated isoleucine transport assay.\nEvidence access: Primary full text\nBCAA catabolism in brown fat controls energy homeostasis through SLC25A44. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31435015/ · DOI 10.1038/s41586-019-1503-x","model_system":"Mouse brown adipocytes/Neuro2a and membrane/proteoliposome assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4b00f118-00fd-5cef-be97-13125eb958e9","stable_key":"import-09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2","title":"L-Isoleucine: transport, translation, catabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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