{"id":"eafab6cb-d288-526a-b64f-073e1a813abf","stable_key":"research:scly-supports-selenop-selenium-reuse","predicate":"supports","statement":"SCLY knockdown reduced incorporation of SELENOP-derived selenium into newly synthesized selenoproteins in HeLa cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"positive","is_public":true,"mechanism_event_id":"90b77023-7bb2-5dd2-956a-5a1b7c285a7f","mechanism_event_label":"These cells needed SCLY for efficient selenium reuse.","subject":{"id":"7c1bfe9f-559b-598e-9cca-8d0b032919fd","slug":"scly","display_name":"SCLY","entity_type_key":"protein"},"object":{"id":"f4969f01-dda8-5b68-8352-07a92a6cfb50","slug":"selenoprotein-biosynthesis","display_name":"Selenoprotein biosynthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"90b77023-7bb2-5dd2-956a-5a1b7c285a7f","stable_key":"research:scly-supports-selenop-selenium-reuse","event_type":"genetic-perturbation","label":"These cells needed SCLY for efficient selenium reuse.","description":"SCLY knockdown reduced incorporation of SELENOP-derived selenium into newly synthesized selenoproteins in HeLa cells.","status":"active","compartment":null,"participants":[{"entity":{"id":"7c1bfe9f-559b-598e-9cca-8d0b032919fd","slug":"scly","display_name":"SCLY","entity_type_key":"protein"},"role":"perturbed-protein","stoichiometry":null,"state_label":"siRNA depletion","sequence_order":0,"notes":""},{"entity":{"id":"c7fbd153-6444-50b2-a8be-6936e352a11c","slug":"selenop","display_name":"SELENOP","entity_type_key":"protein"},"role":"selenium-source","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f4969f01-dda8-5b68-8352-07a92a6cfb50","slug":"selenoprotein-biosynthesis","display_name":"Selenoprotein biosynthesis","entity_type_key":"cellular_process"},"role":"measured-process","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"HeLa","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"75Se-labeled SELENOP and RNA interference","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-specific dependency; not a universal uptake sequence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"human","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3b1e120f-f6c8-55c1-ac9d-da2430f4a4ae","evidence_kind":"curated_literature_summary","locator":"lines 534-544","start_line":534,"end_line":544,"excerpt":"## scly-supports-selenop-selenium-reuse\n\nThese cells needed SCLY for efficient selenium reuse.\n\nSCLY knockdown reduced incorporation of SELENOP-derived selenium into newly synthesized selenoproteins in HeLa cells.\n\nOrganism: human\nCell type: HeLa\nExperimental model: 75Se-labeled SELENOP and RNA interference\nLimitations: Cell-specific dependency; not a universal uptake sequence.\nPrimary reference: [Mammalian Selenocysteine Lyase Is Involved in Selenoprotein Biosynthesis](https://www.jstage.jst.go.jp/article/jnsv/57/4/57_4_298/_article/-char/en)","model_system":"75Se-labeled SELENOP and RNA interference","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"selenium-recycling","plain_language":"These cells needed SCLY for efficient selenium reuse.","evidence_scope":"direct_experimental","papers":[{"key":"kurokawa-2011-scly-recycling","title":"Mammalian Selenocysteine Lyase Is Involved in Selenoprotein Biosynthesis","url":"https://www.jstage.jst.go.jp/article/jnsv/57/4/57_4_298/_article/-char/en","doi":"10.3177/jnsv.57.298","year":2011,"model":"Human HeLa RNA interference and selenium-labeling experiments","summary":"SCLY knockdown reduces reuse of SELENOP-derived selenium for new selenoproteins."}]}}