{"id":"eae5d282-2976-5352-b04c-747af69b07e9","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:preparation-scope-elderberry-extracts-mladenka-2016","predicate":"present_in_specified_preparation","statement":"The specified preparation contains anthocyanins; its experimental effects remain attached to the actual preparation. ","claim_class":"identity","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"c3ea65d8-0a5f-5425-8eac-97a4330f4f1f","mechanism_event_label":"The specified preparation contains anthocyanins; its experimental effects remain attached to the actual preparation. ","subject":{"id":"a1b1c9c3-cafe-5dd6-a590-f56222d3a4cc","slug":"anthocyanins","display_name":"Anthocyanins","entity_type_key":"chemical_species"},"object":{"id":"e00a80c5-8b02-5a86-a3e7-c2acf829d870","slug":"elderberry-extracts-mladenka-2016","display_name":"Ten standardized elderberry fruit extracts in Mladenka 2016","entity_type_key":"botanical"},"evidence_count":1,"mechanism_event":{"id":"c3ea65d8-0a5f-5425-8eac-97a4330f4f1f","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:preparation-scope-elderberry-extracts-mladenka-2016-event","event_type":"preparation_composition","label":"The specified preparation contains anthocyanins; its experimental effects remain attached to the actual preparation. ","description":"**Iron binding and metal reduction are different reactions.** Purified cyanidin, C3G and standardized elderberry extracts chelated iron and reduced iron and copper in cell-free assays; copper-chelating activity was relatively low. Extract behavior varied and was not completely explained by pigment concentration. Binding a metal, changing its oxidation state and reducing intestinal absorption are three different endpoints. This does not establish removal of intracellular metals or protection from ferroptosis in people. [Mladěnka et al., 2016](https://doi.org/10.1016/j.phytol.2016.10.025).","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a1b1c9c3-cafe-5dd6-a590-f56222d3a4cc","slug":"anthocyanins","display_name":"Anthocyanins","entity_type_key":"chemical_species"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"e00a80c5-8b02-5a86-a3e7-c2acf829d870","slug":"elderberry-extracts-mladenka-2016","display_name":"Ten standardized elderberry fruit extracts in Mladenka 2016","entity_type_key":"botanical"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary article abstract read in indexed publisher/author-hosted result; publisher full text unavailable. Exact pH series and extract/metal ratios not extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Preparation identity/scope; not isolated class causation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A food or extract contains other constituents. Membership is a navigation link and does not transfer its findings to isolated anthocyanins.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The specified preparation contains anthocyanins; its experimental effects remain attached to the actual preparation. ","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Fruit extracts of 10 varieties of elderberry (Sambucus nigra L.) interact differently with iron and copper | 2016 | DOI 10.1016/j.phytol.2016.10.025 | https://doi.org/10.1016/j.phytol.2016.10.025","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 88-88; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"42f756a5-fb48-50d2-9962-0d906a5a4967","evidence_kind":"source_excerpt","locator":"Lines 88-88","start_line":88,"end_line":88,"excerpt":"**Iron binding and metal reduction are different reactions.** Purified cyanidin, C3G and standardized elderberry extracts chelated iron and reduced iron and copper in cell-free assays; copper-chelating activity was relatively low. Extract behavior varied and was not completely explained by pigment concentration. Binding a metal, changing its oxidation state and reducing intestinal absorption are three different endpoints. This does not establish removal of intracellular metals or protection from ferroptosis in people. [Mladěnka et al., 2016](https://doi.org/10.1016/j.phytol.2016.10.025).","model_system":"Preparation identity/scope; not isolated class causation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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