{"id":"ea6190d9-664f-588a-999b-d8fff32202ab","stable_key":"c94a9824-35fb-523c-b421-dc7341218dcb:indicaxanthin-dpi-pgd2-blockade","predicate":"prevents_indicaxanthin_increase","statement":"Adding DPI prevented the indicaxanthin-associated increase in PGD2 production in LPS-stimulated mouse macrophages.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"452d1c4e-a43f-5650-905f-dc97421582df","mechanism_event_label":"Adding DPI prevented the indicaxanthin-associated increase in PGD2 production in LPS-stimulated mouse macrophages.","subject":{"id":"cb94492a-d56e-54a7-9bc1-c229446c368c","slug":"diphenyleneiodonium","display_name":"Diphenyleneiodonium / DPI","entity_type_key":"small_molecule"},"object":{"id":"327bf30e-7a1e-544c-b7e2-1e1e773125cc","slug":"mouse-raw2647-pgd2-production","display_name":"Prostaglandin D2 production in mouse RAW 264.7 macrophages","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"452d1c4e-a43f-5650-905f-dc97421582df","stable_key":"c94a9824-35fb-523c-b421-dc7341218dcb:indicaxanthin-dpi-pgd2-blockade-event","event_type":"observed_relationship","label":"Adding DPI prevented the indicaxanthin-associated increase in PGD2 production in LPS-stimulated mouse macrophages.","description":"Adding DPI prevented the indicaxanthin-associated increase in PGD2 production in LPS-stimulated mouse macrophages.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cb94492a-d56e-54a7-9bc1-c229446c368c","slug":"diphenyleneiodonium","display_name":"Diphenyleneiodonium / DPI","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"327bf30e-7a1e-544c-b7e2-1e1e773125cc","slug":"mouse-raw2647-pgd2-production","display_name":"Prostaglandin D2 production in mouse RAW 264.7 macrophages","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f2a6e469-05f0-5596-9054-da926389f584","slug":"indicaxanthin","display_name":"Indicaxanthin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"51dae53e-ef81-5060-98e8-b1a519819cb6","slug":"prostaglandin-d2","display_name":"Prostaglandin D2","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"dose","value_text":"Indicaxanthin 50-100 micromolar; DPI 1 micromolar; LPS 1 microgram/mL","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Indicaxanthin 1 h before LPS; PGD2 at 8 h","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary open full text, relevant results/methods and PubMed metadata.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"LPS-stimulated mouse RAW 264.7 macrophages with DPI exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"DPI is not NOX-isoform-selective; pharmacological sensitivity does not establish NOX2 necessity or a nutrient deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Dedicated indicaxanthin chapter; original betalain family identity and shared claims preserved.","comparator":null,"unit":null,"notes":"","entity":{"slug":"indicaxanthin","display_name":"Indicaxanthin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"LPS-stimulated mouse RAW 264.7 macrophages with DPI exposure","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Adding DPI prevented the indicaxanthin-associated increase in PGD2 production in LPS-stimulated mouse macrophages.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Pro-oxidant activity of indicaxanthin from Opuntia ficus indica modulates arachidonate metabolism and prostaglandin synthesis through lipid peroxide production in LPS-stimulated RAW 264.7 macrophages. (2014). https://pubmed.ncbi.nlm.nih.gov/25180166/ DOI: 10.1016/j.redox.2014.07.004","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro co-incubation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Redox-dependent prostaglandin pathway","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"401f210a-22dc-5acc-aa63-e69e45564775","evidence_kind":"source_excerpt","locator":"Lines 184-193","start_line":184,"end_line":193,"excerpt":"## indicaxanthin-dpi-pgd2-blockade\nAdding DPI prevented the indicaxanthin-associated increase in PGD2 production in LPS-stimulated mouse macrophages.\nModel/species: LPS-stimulated mouse RAW 264.7 macrophages with DPI exposure\nTissue: Redox-dependent prostaglandin pathway\nExposure: Indicaxanthin 50-100 micromolar; DPI 1 micromolar; LPS 1 microgram/mL\nRoute: In vitro co-incubation\nDuration: Indicaxanthin 1 h before LPS; PGD2 at 8 h\nLimits: DPI is not NOX-isoform-selective; pharmacological sensitivity does not establish NOX2 necessity or a nutrient deficiency.\nPrimary reference: Pro-oxidant activity of indicaxanthin from Opuntia ficus indica modulates arachidonate metabolism and prostaglandin synthesis through lipid peroxide production in LPS-stimulated RAW 264.7 macrophages. (2014). https://pubmed.ncbi.nlm.nih.gov/25180166/ DOI: 10.1016/j.redox.2014.07.004\nAccess: Primary open full text, relevant results/methods and PubMed metadata.","model_system":"LPS-stimulated mouse RAW 264.7 macrophages with DPI exposure","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact archived curation span; original paraphrase with primary citation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"18b7303c-98b7-54cb-ae8e-a8c6257657b1","stable_key":"import-c94a9824-35fb-523c-b421-dc7341218dcb","title":"Indicaxanthin: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of thirteen additional primary studies, with twenty-six existing claims from eight studies linked unchanged. Study-specific citations and limitations retained. 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