{"id":"ea56bb1e-e132-59dc-92c3-0d278c75f53a","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-adipocyte-promoter","predicate":"reduces_promoter_occupancy","statement":"Fisetin reduced C/EBP-alpha occupancy at the GLUT4 promoter in mouse 3T3-L1 cells, alongside reduced mTOR/S6K signaling.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"cab9bb9a-fa34-585f-8a4f-96d8868b17ae","mechanism_event_label":"A transcriptional step links signaling to glucose transport.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"420287ec-8f83-55b0-b42b-f87358dfd0f0","slug":"mouse-cebpa","display_name":"Mouse C/EBP alpha / Cebpa","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"cab9bb9a-fa34-585f-8a4f-96d8868b17ae","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-adipocyte-promoter-event","event_type":"observed_relationship","label":"A transcriptional step links signaling to glucose transport.","description":"Fisetin reduced C/EBP-alpha occupancy at the GLUT4 promoter in mouse 3T3-L1 cells, alongside reduced mTOR/S6K signaling.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"420287ec-8f83-55b0-b42b-f87358dfd0f0","slug":"mouse-cebpa","display_name":"Mouse C/EBP alpha / Cebpa","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"66d34dc0-d0fc-5ebb-9d7c-dc88f6b47cea","slug":"mouse-slc2a4","display_name":"Mouse glucose transporter 4 / Slc2a4","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2bf56761-aaca-5d23-aadc-5d1f80178ec4","slug":"mouse-mtor","display_name":"Mouse mechanistic target of rapamycin / Mtor","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"763c166a-163a-5c5c-b136-2890efa8d822","slug":"mouse-rps6kb1","display_name":"Mouse S6 kinase 1 / Rps6kb1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Chromatin immunoprecipitation; comparison with rapamycin.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Association and phenocopy do not resolve every direct target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A transcriptional step links signaling to glucose transport.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Fisetin Suppresses Lipid Accumulation in Mouse Adipocytic 3T3-L1 Cells by Repressing GLUT4-Mediated Glucose Uptake through Inhibition of mTOR-C/EBPα Signaling. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25945786/ · DOI 10.1021/acs.jafc.5b00821","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"aa64e06b-9358-5180-81d4-04377eb6f32a","evidence_kind":"source_excerpt","locator":"Lines 304-310","start_line":304,"end_line":310,"excerpt":"## fisetin-adipocyte-promoter\nA transcriptional step links signaling to glucose transport.\nFisetin reduced C/EBP-alpha occupancy at the GLUT4 promoter in mouse 3T3-L1 cells, alongside reduced mTOR/S6K signaling.\nModel: Chromatin immunoprecipitation; comparison with rapamycin.\nLimitations: Association and phenocopy do not resolve every direct target.\nEvidence access: Primary abstract\nFisetin Suppresses Lipid Accumulation in Mouse Adipocytic 3T3-L1 Cells by Repressing GLUT4-Mediated Glucose Uptake through Inhibition of mTOR-C/EBPα Signaling. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25945786/ · DOI 10.1021/acs.jafc.5b00821","model_system":"Chromatin immunoprecipitation; comparison with rapamycin.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}