{"id":"ea400582-ed04-59fa-accc-5319e6857ae3","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-inpp1-magnesium","predicate":"activates","statement":"Purified calf-brain INPP1 showed cooperative magnesium dependence, with half-maximal stimulation near 0.3 mM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8d7b9c71-f45d-5394-900c-42665e8621c7","mechanism_event_label":"A second recycling enzyme also depends on magnesium.","subject":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"object":{"id":"49a701d3-dc96-5389-bafd-6948f38dc393","slug":"bovine-inpp1","display_name":"Bovine inositol polyphosphate 1-phosphatase / INPP1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"8d7b9c71-f45d-5394-900c-42665e8621c7","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-inpp1-magnesium-event","event_type":"observed_relationship","label":"A second recycling enzyme also depends on magnesium.","description":"Purified calf-brain INPP1 showed cooperative magnesium dependence, with half-maximal stimulation near 0.3 mM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"49a701d3-dc96-5389-bafd-6948f38dc393","slug":"bovine-inpp1","display_name":"Bovine inositol polyphosphate 1-phosphatase / INPP1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e2e3902-5e26-52e3-9c85-1d7c0ace1637","slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"5b09f2d6-d3ce-50ee-80d7-a77680b379a2","slug":"inositol-1-4-bisphosphate","display_name":"Myo-inositol 1,4-bisphosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"7717d93b-5b8d-591a-a0ae-d7ee859f826d","slug":"inositol-1-3-4-trisphosphate","display_name":"Myo-inositol 1,3,4-trisphosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Calf-brain enzyme purification and kinetics.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"In-vitro cofactor concentration is not a human blood target.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lithium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"A second recycling enzyme also depends on magnesium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inositol polyphosphate 1-phosphatase from calf brain. Purification and inhibition by Li+, Ca2+, and Mn2+. · 1987 · https://pubmed.ncbi.nlm.nih.gov/2824473/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fb1cfd20-2ff6-5b94-8384-21c9a17ba8c8","evidence_kind":"source_excerpt","locator":"Lines 48-54","start_line":48,"end_line":54,"excerpt":"## lithium-inpp1-magnesium\nA second recycling enzyme also depends on magnesium.\nPurified calf-brain INPP1 showed cooperative magnesium dependence, with half-maximal stimulation near 0.3 mM.\nModel: Calf-brain enzyme purification and kinetics.\nLimitations: In-vitro cofactor concentration is not a human blood target.\nEvidence access: Primary abstract\nInositol polyphosphate 1-phosphatase from calf brain. Purification and inhibition by Li+, Ca2+, and Mn2+. · 1987 · https://pubmed.ncbi.nlm.nih.gov/2824473/","model_system":"Calf-brain enzyme purification and kinetics.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b2dd2f3c-5c4c-5a12-9a0d-91b521ffcf98","stable_key":"import-b7798a90-72a3-5454-a321-c4be88bf0cc4","title":"Lithium: metal-sensitive enzymes, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"dc67e965ba78458cf2d88d000d687641d9104354115ab16529a3a64941bd777d","revision_id":"4af127bf-9ccc-516b-9481-fafd453c2c35","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}