{"id":"ea1f19e8-e3de-5b48-bac1-2e113d521104","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-ampk-alt1-dependence","predicate":"supports_alanine_response","statement":"ALT1 siRNA reduced rat H4IIE ALT protein by about 77% and reduced alanine-stimulated AMPK activation by about 49%.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"51fdc9ef-9060-559e-885c-b20ea4dafe5d","mechanism_event_label":"The response partly depended on processing alanine, rather than simply sensing it outside the cell.","subject":{"id":"fecc5017-f6e3-58cc-8057-c26d35b47054","slug":"rat-gpt","display_name":"Rat cytosolic alanine aminotransferase / Gpt","entity_type_key":"protein"},"object":{"id":"2f112afe-1ecd-54a0-a90d-a8c6ac5cecb6","slug":"rat-ampk-complex","display_name":"Rat AMP-activated protein kinase complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"51fdc9ef-9060-559e-885c-b20ea4dafe5d","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-ampk-alt1-dependence-event","event_type":"observed_relationship","label":"The response partly depended on processing alanine, rather than simply sensing it outside the cell.","description":"ALT1 siRNA reduced rat H4IIE ALT protein by about 77% and reduced alanine-stimulated AMPK activation by about 49%.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fecc5017-f6e3-58cc-8057-c26d35b47054","slug":"rat-gpt","display_name":"Rat cytosolic alanine aminotransferase / Gpt","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2f112afe-1ecd-54a0-a90d-a8c6ac5cecb6","slug":"rat-ampk-complex","display_name":"Rat AMP-activated protein kinase complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"91e6d4f5-fba1-542d-b68a-57cc28e1e425","slug":"pyruvate","display_name":"Pyruvate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"628ddc2d-e4d9-59c7-ae76-0c0cab80a6b1","slug":"2-oxoglutarate","display_name":"2-Oxoglutarate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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