{"id":"ea0f47e7-da88-5475-aefc-2e48441705e8","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-agmat-reannotation","predicate":"does_not_hydrolyze_in_assay","statement":"Purified recombinant human AGMAT variants R105 and G105 showed no detected agmatine hydrolysis at 10 mM substrate in the 2022 assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"f2bc5e53-62d5-5c90-9b65-854029f3a6d0","mechanism_event_label":"A gene name does not guarantee the biochemical reaction usually drawn beside it.","subject":{"id":"bba88813-506c-5bbf-b85e-fc33beefc748","slug":"agmat","display_name":"Human AGMAT, guanidino acid hydrolase candidate / historically annotated agmatinase","entity_type_key":"protein"},"object":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"f2bc5e53-62d5-5c90-9b65-854029f3a6d0","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-agmat-reannotation-event","event_type":"observed_relationship","label":"A gene name does not guarantee the biochemical reaction usually drawn beside it.","description":"Purified recombinant human AGMAT variants R105 and G105 showed no detected agmatine hydrolysis at 10 mM substrate in the 2022 assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bba88813-506c-5bbf-b85e-fc33beefc748","slug":"agmat","display_name":"Human AGMAT, guanidino acid hydrolase candidate / historically annotated agmatinase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text; Figures 1 and 2","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human proteins expressed in E. coli; urea assay, detection limit about 0.02 nmol/s/mg.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This assay result revises the proposed substrate assignment; it does not prove that humans cannot metabolize agmatine by other routes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A gene name does not guarantee the biochemical reaction usually drawn beside it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Guanidino acid hydrolysis by the human enzyme annotated as agmatinase. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36543883/ · DOI 10.1038/s41598-022-26655-4","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9d0b8bba-280d-57b4-9490-d65bd30d260b","evidence_kind":"source_excerpt","locator":"Lines 116-122","start_line":116,"end_line":122,"excerpt":"## agmatine-sulfate-agmat-reannotation\nA gene name does not guarantee the biochemical reaction usually drawn beside it.\nPurified recombinant human AGMAT variants R105 and G105 showed no detected agmatine hydrolysis at 10 mM substrate in the 2022 assay.\nModel: Human proteins expressed in E. coli; urea assay, detection limit about 0.02 nmol/s/mg.\nLimitations: This assay result revises the proposed substrate assignment; it does not prove that humans cannot metabolize agmatine by other routes.\nEvidence access: Primary full text; Figures 1 and 2\nGuanidino acid hydrolysis by the human enzyme annotated as agmatinase. · 2022 · https://pubmed.ncbi.nlm.nih.gov/36543883/ · DOI 10.1038/s41598-022-26655-4","model_system":"Human proteins expressed in E. coli; urea assay, detection limit about 0.02 nmol/s/mg.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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