{"id":"ea0eb271-a909-5c3f-851e-ae3272e29419","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-nadk2-proline","predicate":"supports_reductive_synthesis_of","statement":"NADK2 deletion reduced glutamine-derived proline synthesis; mitochondrial NADP(H) was needed for the P5CS step reducing glutamate toward P5C.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"374fb7da-4ffe-5ce1-a27b-bca80aba331d","mechanism_event_label":"Proline production needs reducing power in the right compartment.","subject":{"id":"eac1269b-354c-52d4-b3e6-26e6cd111632","slug":"nadk2","display_name":"NADK2","entity_type_key":"protein"},"object":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"374fb7da-4ffe-5ce1-a27b-bca80aba331d","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-nadk2-proline-event","event_type":"observed_relationship","label":"Proline production needs reducing power in the right compartment.","description":"NADK2 deletion reduced glutamine-derived proline synthesis; 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unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cell deletion, isotope tracing and rescue experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The limiting step was cofactor-dependent synthesis, not a demonstrated lack of glutamine in the diet.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Proline collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Proline production needs reducing power in the right compartment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Mitochondrial NADP(H) generation is essential for proline biosynthesis. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33888598/ · DOI 10.1126/science.abd5491","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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primary references, access levels and experimental limitations individually identified. 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