{"id":"e9fabb97-0535-5619-a327-c34db0f72c2f","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-pn-enzyme-inhibition","predicate":"inhibits","statement":"Pyridoxine inhibited the tested PLP-dependent bacterial tyrosine decarboxylase preparation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"265233b9-8db5-536f-a998-c0950bfbe1b0","mechanism_event_label":"Pyridoxine interfered with one isolated B6-dependent enzyme.","subject":{"id":"aa6648f2-8962-5567-9939-ef4145e40ed1","slug":"pyridoxine","display_name":"Pyridoxine","entity_type_key":"small_molecule"},"object":{"id":"e8f2d674-296d-5160-9e98-c423f754ff59","slug":"enterococcus-faecalis-tyrosine-decarboxylase","display_name":"Enterococcus faecalis tyrosine decarboxylase","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"265233b9-8db5-536f-a998-c0950bfbe1b0","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-neuro-pn-enzyme-inhibition-event","event_type":"biochemical_relationship","label":"Pyridoxine interfered with one isolated B6-dependent enzyme.","description":"Pyridoxine inhibited the tested PLP-dependent bacterial tyrosine decarboxylase preparation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"aa6648f2-8962-5567-9939-ef4145e40ed1","slug":"pyridoxine","display_name":"Pyridoxine","entity_type_key":"small_molecule"},"role":"inhibitor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e8f2d674-296d-5160-9e98-c423f754ff59","slug":"enterococcus-faecalis-tyrosine-decarboxylase","display_name":"Enterococcus faecalis tyrosine decarboxylase","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"cofactor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Differentiated human SH-SY5Y and CaCo-2 cells; isolated enzyme assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"5 micromolar pyridoxine; commercial Streptococcus faecalis enzyme (current Enterococcus name).","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure-class","value_text":"Experimental excess pyridoxine; not dietary B6 deficiency","comparator":null,"unit":null,"notes":"","entity":{"slug":"pyridoxine","display_name":"Pyridoxine","entity_type_key":"small_molecule"}},{"dimension":"limitations","value_text":"Not human GAD, DDC or PDXK; mechanism of human neuropathy remains unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Enterococcus faecalis (reported as Streptococcus faecalis)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Pyridoxine interfered with one isolated B6-dependent enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[vrolijk-2017-pn] The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function (2017). https://doi.org/10.1016/j.tiv.2017.07.009 DOI: 10.1016/j.tiv.2017.07.009","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell-free bacterial enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"47db67cf-63b0-52e8-a9c7-fa30ea5cfe17","evidence_kind":"source_excerpt","locator":"Lines 1258-1269","start_line":1258,"end_line":1269,"excerpt":"### b6-neuro-pn-enzyme-inhibition\nPyridoxine inhibited the tested PLP-dependent bacterial tyrosine decarboxylase preparation.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Pyridoxine interfered with one isolated B6-dependent enzyme.\norganism: Enterococcus faecalis (reported as Streptococcus faecalis)\ntissue_or_cell_type: Cell-free bacterial enzyme\nexperimental_model: Differentiated human SH-SY5Y and CaCo-2 cells; isolated enzyme assays\nlimitations: Not human GAD, DDC or PDXK; mechanism of human neuropathy remains unresolved.\nexposure: 5 micromolar pyridoxine; commercial Streptococcus faecalis enzyme (current Enterococcus name).\nexposure-class: Experimental excess pyridoxine; not dietary B6 deficiency\n[vrolijk-2017-pn] The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function (2017). https://doi.org/10.1016/j.tiv.2017.07.009 DOI: 10.1016/j.tiv.2017.07.009","model_system":"Differentiated human SH-SY5Y and CaCo-2 cells; isolated enzyme assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [vrolijk-2017-pn] The vitamin B6 paradox: Supplementation with high concentrations of pyridoxine leads to decreased vitamin B6 function (2017). https://doi.org/10.1016/j.tiv.2017.07.009 DOI: 10.1016/j.tiv.2017.07.009","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}