{"id":"e9a8dc8f-c8bc-581c-b832-a02dce7a4ce8","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-sig-atra-rxr-selectivity","predicate":"does-not-effectively-compete-for","statement":"All-trans RA did not effectively compete for RXR binding in the tested isomer assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"49b4d03d-25b0-5596-a6fc-6ddc39fb1ef4","mechanism_event_label":"RAR and RXR cannot be treated as interchangeable RA receptors.","subject":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"object":{"id":"bc49fbd3-6a7d-5cc4-9e4a-57aa0fa31928","slug":"rxr-family","display_name":"Retinoid X receptor family","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"49b4d03d-25b0-5596-a6fc-6ddc39fb1ef4","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-sig-atra-rxr-selectivity-event","event_type":"biochemical_relationship","label":"RAR and RXR cannot be treated as interchangeable RA receptors.","description":"All-trans RA did not effectively compete for RXR binding in the tested isomer assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ce392c9e-6483-5914-b18b-d6a2520dbbf6","slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"},"role":"nutrient-context","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7dc1ce11-f208-5187-95d9-96b07927df6a","slug":"rxra","display_name":"Retinoid X receptor alpha","entity_type_key":"protein"},"role":"tested-subtype","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"64e5717c-8c8a-59ee-b67c-66ae86ac376b","slug":"rxrb","display_name":"Retinoid X receptor beta","entity_type_key":"protein"},"role":"tested-subtype","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b452b24e-f0a3-5285-884d-c19fe3235020","slug":"rxrg","display_name":"Retinoid X receptor gamma","entity_type_key":"protein"},"role":"tested-subtype","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"bc49fbd3-6a7d-5cc4-9e4a-57aa0fa31928","slug":"rxr-family","display_name":"Retinoid X receptor family","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_locator","value_text":"Abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Transfected COS-1 nucleosol; ligand competition and chimeric receptor reporters.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Isomer competition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell metabolism can alter apparent reporter responses.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Recombinant receptors in COS-1 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"RAR and RXR cannot be treated as interchangeable RA receptors.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[va-allenby-1993] Retinoic acid receptors and retinoid X receptors: interactions with endogenous retinoic acids (1993). https://pubmed.ncbi.nlm.nih.gov/8380496/ DOI: 10.1073/pnas.90.1.30","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Nuclear extracts","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b9002f5b-3eda-500e-b269-e4505626be38","evidence_kind":"source_excerpt","locator":"Lines 1056-1067","start_line":1056,"end_line":1067,"excerpt":"### va-sig-atra-rxr-selectivity\nAll-trans RA did not effectively compete for RXR binding in the tested isomer assay.\nCondition category: normal\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: RAR and RXR cannot be treated as interchangeable RA receptors.\norganism: Recombinant receptors in COS-1 cells\ntissue_or_cell_type: Nuclear extracts\nexperimental_model: Transfected COS-1 nucleosol; ligand competition and chimeric receptor reporters.\nlimitations: Cell metabolism can alter apparent reporter responses.\nevidence_locator: Abstract\nexposure: Isomer competition\n[va-allenby-1993] Retinoic acid receptors and retinoid X receptors: interactions with endogenous retinoic acids (1993). https://pubmed.ncbi.nlm.nih.gov/8380496/ DOI: 10.1073/pnas.90.1.30","model_system":"Transfected COS-1 nucleosol; ligand competition and chimeric receptor reporters.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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