{"id":"e9524f87-16aa-5ac9-8c6f-d6e8c0003471","stable_key":"d39c4b36-9a88-5f52-8e6b-93a248dc4bfc:hericenones-erinacines-extract-ngf-synergy","predicate":"potentiates","statement":"One microgram/mL aqueous Hericium extract combined with 10 ng/mL NGF produced the largest neurite-outgrowth response, while the extract did not protect against the oxidative-stress challenge.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"62b92c70-49a5-5ac1-afb9-bee031872883","mechanism_event_label":"One microgram/mL aqueous Hericium extract combined with 10 ng/mL NGF produced the largest neurite-outgrowth response, while the extract did not protect against the oxidative-stress challenge.","subject":{"id":"be06b917-d388-570b-9031-fa3e914ee180","slug":"hericium-aqueous-extract-2013","display_name":"Hericium erinaceus aqueous extract used in the 2013 neurite study","entity_type_key":"botanical"},"object":{"id":"7ea9698c-a904-5071-a9c9-9621e2741faa","slug":"ngf-induced-ng10815-neurite-outgrowth","display_name":"NGF-induced neurite outgrowth in NG108-15 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"62b92c70-49a5-5ac1-afb9-bee031872883","stable_key":"d39c4b36-9a88-5f52-8e6b-93a248dc4bfc:hericenones-erinacines-extract-ngf-synergy-event","event_type":"observed_relationship","label":"One microgram/mL aqueous Hericium extract combined with 10 ng/mL NGF produced the largest neurite-outgrowth response, while the extract did not protect against the oxidative-stress challenge.","description":"One microgram/mL aqueous Hericium extract combined with 10 ng/mL NGF produced the largest neurite-outgrowth response, while the extract did not protect against the oxidative-stress challenge.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"be06b917-d388-570b-9031-fa3e914ee180","slug":"hericium-aqueous-extract-2013","display_name":"Hericium erinaceus aqueous extract used in the 2013 neurite study","entity_type_key":"botanical"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7ea9698c-a904-5071-a9c9-9621e2741faa","slug":"ngf-induced-ng10815-neurite-outgrowth","display_name":"NGF-induced neurite outgrowth in NG108-15 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"05f59de8-50e8-5e30-b0b2-3b5812b022f7","slug":"human-ngf","display_name":"Human nerve growth factor / NGF","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Aqueous extract 1 microgram/mL with NGF 10 ng/mL","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Cell differentiation interval","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"NG108-15 neuroblastoma-glioma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A whole aqueous extract plus exogenous NGF is not evidence for a specific hericenone or erinacine, and the oxidative-stress result was null.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Hericenones & Erinacines chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"hericenones-erinacines","display_name":"Hericenones and erinacines","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"NG108-15 neuroblastoma-glioma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"One microgram/mL aqueous Hericium extract combined with 10 ng/mL NGF produced the largest neurite-outgrowth response, while the extract did not protect against the oxidative-stress challenge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Neurotrophic properties of the Lion's mane medicinal mushroom, Hericium erinaceus (Higher Basidiomycetes) from Malaysia. (2013). https://pubmed.ncbi.nlm.nih.gov/24266378/ DOI: 10.1615/intjmedmushr.v15.i6.30","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Neurite outgrowth and oxidative-stress survival","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5f18cae4-712a-5eb1-abed-26173357f87b","evidence_kind":"source_excerpt","locator":"Lines 33-42","start_line":33,"end_line":42,"excerpt":"## hericenones-erinacines-extract-ngf-synergy\nOne microgram/mL aqueous Hericium extract combined with 10 ng/mL NGF produced the largest neurite-outgrowth response, while the extract did not protect against the oxidative-stress challenge.\nModel/species: NG108-15 neuroblastoma-glioma cells\nTissue/system: Neurite outgrowth and oxidative-stress survival\nExposure: Aqueous extract 1 microgram/mL with NGF 10 ng/mL\nRoute: In vitro\nDuration: Cell differentiation interval\nLimits: A whole aqueous extract plus exogenous NGF is not evidence for a specific hericenone or erinacine, and the oxidative-stress result was null.\nPrimary reference: Neurotrophic properties of the Lion's mane medicinal mushroom, Hericium erinaceus (Higher Basidiomycetes) from Malaysia. (2013). https://pubmed.ncbi.nlm.nih.gov/24266378/ DOI: 10.1615/intjmedmushr.v15.i6.30\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"NG108-15 neuroblastoma-glioma cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"aa7c09fd-ab4a-51ff-b463-d35547dc41ba","stable_key":"import-d39c4b36-9a88-5f52-8e6b-93a248dc4bfc","title":"Hericenones & Erinacines: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. 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