{"id":"e942bcea-67ca-5196-9066-9e7b6618f6c4","stable_key":"33bef151-bdc7-5892-aff6-0345ab149739:a-single-oral-dose-lowers-factor-viii-activity","predicate":"reduces","statement":"Factor VIII activity declined significantly at 4 and 6 h after the same single dose.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"cf50da41-52a0-5bf9-a4ee-f63aa125394f","mechanism_event_label":"Factor VIII activity declined significantly at 4 and 6 h after the same single dose.","subject":{"id":"9910223c-2a27-5d75-8003-91d72e71be9a","slug":"nsk-sd","display_name":"NSK-SD fermented soybean extract","entity_type_key":"chemical_species"},"object":{"id":"87d8ad0b-abcc-5313-8aa4-985eff05e6dd","slug":"f8","display_name":"Human coagulation factor VIII / F8","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"cf50da41-52a0-5bf9-a4ee-f63aa125394f","stable_key":"33bef151-bdc7-5892-aff6-0345ab149739:a-single-oral-dose-lowers-factor-viii-activity-event","event_type":"observed_intervention","label":"Factor VIII activity declined significantly at 4 and 6 h after the same single dose.","description":"Plasma","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9910223c-2a27-5d75-8003-91d72e71be9a","slug":"nsk-sd","display_name":"NSK-SD fermented soybean extract","entity_type_key":"chemical_species"},"role":"acting component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"87d8ad0b-abcc-5313-8aa4-985eff05e6dd","slug":"f8","display_name":"Human coagulation factor VIII / F8","entity_type_key":"protein"},"role":"component the finding is about","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Single dose, sampling to 8 h","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human (12 healthy young males)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"NSK-SD, 2,000 FU single dose","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Within the normal range. No direct proteolytic cleavage of factor VIII by purified nattokinase has been shown anywhere in the retrieved evidence, so the step producing this is unidentified.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human (12 healthy young males)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Factor VIII activity declined significantly at 4 and 6 h after the same single dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A single-dose of oral nattokinase potentiates thrombolysis and anti-coagulation profiles. (2015) https://pubmed.ncbi.nlm.nih.gov/26109079/ DOI: 10.1038/srep11601","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vivo, oral","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Plasma","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bf1ba117-47d7-573b-a9a7-308180da21e0","evidence_kind":"source_excerpt","locator":"Lines 873-873","start_line":873,"end_line":873,"excerpt":"Factor VIII activity declined significantly at 4 and 6 h after the same single dose.","model_system":"Human (12 healthy young males)","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"41cb127c-1c23-53aa-b7d0-5ec7f6fa3c99","stable_key":"import-33bef151-bdc7-5892-aff6-0345ab149739","title":"Nattokinase: what the purified enzyme cleaves, what survives being eaten, and the gap between the two (2026-09-23)","document_type":"imported_text","citation_label":"Original AI-assisted curation built from a supplied entity-first document of 105 entities and 129 claims. Every reference in that document was resolved against live PubMed with its abstract read and its DOI cross-checked on 2026-09-23, and the EFSA novel-food opinion was retrieved and read in full. That check corrected two PMIDs that pointed at unrelated papers, two DOIs, and two papers recorded as carrying no erratum that do carry one; it also reversed three findings the supplied document had stated backwards. Two papers carry a published correction, recorded as such and not as a retraction. Three sources are not indexed in PubMed and are cited by what they have. Laboratory lineages are recorded, so the four papers from one group, the three from another and the two readings of a single applicant dossier cannot be counted as separate lines of support. Study-specific doses, units, populations and limitations retained; activity units are never converted between systems. Not publisher full text.","file_path":"","sha256":"34fc46e0e849d06bfa13cb099a79b50679dd6fd0de8a5a0575326afdde3259c1","revision_id":"efd063e6-e982-5f6e-a4b8-6f2ffe4185ce","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}