{"id":"e933ba34-903c-54ef-80f5-275686e3da19","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-ap1-double-delivery","predicate":"supports","statement":"Combined loss of AP-1A and AP-1B mu1 subunits reduced trafficking of human NIS to the MDCK cell plasma membrane.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"c45f0aa1-3240-5857-9eac-dc0f21efa190","mechanism_event_label":"Two adaptor systems together support delivery of NIS to the cell surface.","subject":{"id":"2538c97f-a751-5627-b7dd-59286994a146","slug":"canine-ap1a","display_name":"Canine AP-1A clathrin adaptor complex","entity_type_key":"protein_complex"},"object":{"id":"8f3cf8aa-8902-5d9f-ab8f-b204aee1fa9d","slug":"nis-plasma-membrane-delivery","display_name":"NIS delivery to the plasma membrane","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c45f0aa1-3240-5857-9eac-dc0f21efa190","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-ap1-double-delivery-event","event_type":"biochemical_relationship","label":"Two adaptor systems together support delivery of NIS to the cell surface.","description":"Combined loss of AP-1A and AP-1B mu1 subunits reduced trafficking of human NIS to the MDCK cell plasma membrane.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0d051c22-c0d2-5c22-91ad-4ee6894dc7a0","slug":"slc5a5","display_name":"Human sodium/iodide symporter / SLC5A5","entity_type_key":"protein"},"role":"sorted_cargo","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2538c97f-a751-5627-b7dd-59286994a146","slug":"canine-ap1a","display_name":"Canine AP-1A clathrin adaptor complex","entity_type_key":"protein_complex"},"role":"sorting_complex","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d53440b4-da35-5445-9749-638a6a22631e","slug":"canine-ap1b","display_name":"Canine AP-1B clathrin adaptor complex","entity_type_key":"protein_complex"},"role":"compensating_sorting_complex","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8f3cf8aa-8902-5d9f-ab8f-b204aee1fa9d","slug":"nis-plasma-membrane-delivery","display_name":"NIS delivery to the plasma membrane","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human NIS expressed in canine MDCK epithelial cells with AP-1 adaptor subunit silencing","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Combined mu1A/mu1B depletion; comparison with single-subunit depletion and controls.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The experiment concerns protein delivery; it does not identify a dietary cofactor shortage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iodine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human NIS in canine MDCK cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Two adaptor systems together support delivery of NIS to the cell surface.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iodine-trans-sorting2022] Basolateral Sorting of the Sodium/Iodide Symporter Is Mediated by Adaptor Protein 1 Clathrin Adaptor Complexes. (2022). https://pubmed.ncbi.nlm.nih.gov/35833460/ DOI: 10.1089/thy.2022.0163","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Epithelial trans-Golgi/recycling-endosome trafficking","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"ade72d36-3fd6-5dbc-a832-1b501a0f7dc5","evidence_kind":"source_excerpt","locator":"Lines 219-230","start_line":219,"end_line":230,"excerpt":"### iodine-trans-ap1-double-delivery\nCombined loss of AP-1A and AP-1B mu1 subunits reduced trafficking of human NIS to the MDCK cell plasma membrane.\nCondition category: machinery_impairment\nnutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Two adaptor systems together support delivery of NIS to the cell surface.\norganism: Human NIS in canine MDCK cells\ntissue_or_cell_type: Epithelial trans-Golgi/recycling-endosome trafficking\nexperimental_model: Human NIS expressed in canine MDCK epithelial cells with AP-1 adaptor subunit silencing\nlimitations: The experiment concerns protein delivery; it does not identify a dietary cofactor shortage.\nexposure: Combined mu1A/mu1B depletion; comparison with single-subunit depletion and controls.\ncross_nutrient: false\n[iodine-trans-sorting2022] Basolateral Sorting of the Sodium/Iodide Symporter Is Mediated by Adaptor Protein 1 Clathrin Adaptor Complexes. (2022). https://pubmed.ncbi.nlm.nih.gov/35833460/ DOI: 10.1089/thy.2022.0163","model_system":"Human NIS expressed in canine MDCK epithelial cells with AP-1 adaptor subunit silencing","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [iodine-trans-sorting2022] Basolateral Sorting of the Sodium/Iodide Symporter Is Mediated by Adaptor Protein 1 Clathrin Adaptor Complexes. (2022). https://pubmed.ncbi.nlm.nih.gov/35833460/ DOI: 10.1089/thy.2022.0163","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"76da623d-a34a-5a5c-a942-d7571a85c486","stable_key":"import-aaa7baba-8689-56ab-ba1e-b71542bcb8e9","title":"Iodine: thyroid hormone production, deficiency, excess and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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