{"id":"e8113b84-f4fc-5ed2-b835-7b5ff54f99d0","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-nadk2-rescue","predicate":"rescues","statement":"Adding proline restored growth of NADK2-deleted human cells in minimal medium, whereas the defect was largely absent in nutrient-rich medium.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"d077c54c-47cf-5900-ad1e-e75ac55dc42a","mechanism_event_label":"An external supply can bypass a synthesis bottleneck in these cells.","subject":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"object":{"id":"26818bf6-7ae1-5ff6-90c4-a0b81e7bf951","slug":"human-nadk2-null-proliferation","display_name":"Human NADK2-null cell proliferation in minimal medium","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d077c54c-47cf-5900-ad1e-e75ac55dc42a","stable_key":"6612c190-1948-5bcf-bbe3-a7f6c50fa3cf:l-proline-nadk2-rescue-event","event_type":"observed_relationship","label":"An external supply can bypass a synthesis bottleneck in these cells.","description":"Adding proline restored growth of NADK2-deleted human cells in minimal medium, whereas the defect was largely absent in nutrient-rich medium.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"70ccd53c-83ad-5251-a4f5-6370973bc40a","slug":"l-proline","display_name":"L-Proline","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"26818bf6-7ae1-5ff6-90c4-a0b81e7bf951","slug":"human-nadk2-null-proliferation","display_name":"Human NADK2-null cell proliferation in minimal medium","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"eac1269b-354c-52d4-b3e6-26e6cd111632","slug":"nadk2","display_name":"NADK2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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