{"id":"e8022893-9112-5205-b73a-eb2a14c7dd87","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-nicotinic-chick","predicate":"inhibits","statement":"Agmatine at 1 mM reduced DMPP-induced chick retinal depolarization by approximately 70% without preventing neuronal bungarotoxin binding.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"037fadce-e0f4-5f68-b80c-9e903081cb6e","mechanism_event_label":"Nicotinic function was blocked without competing at that toxin-binding site.","subject":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"object":{"id":"66b680c8-26ee-51d6-b3ea-78e96e8350ec","slug":"chick-retinal-nicotinic-depolarization","display_name":"Chick retinal nicotinic depolarization","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"037fadce-e0f4-5f68-b80c-9e903081cb6e","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-nicotinic-chick-event","event_type":"observed_relationship","label":"Nicotinic function was blocked without competing at that toxin-binding site.","description":"Agmatine at 1 mM reduced DMPP-induced chick retinal depolarization by approximately 70% without preventing neuronal bungarotoxin binding.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"66b680c8-26ee-51d6-b3ea-78e96e8350ec","slug":"chick-retinal-nicotinic-depolarization","display_name":"Chick retinal nicotinic depolarization","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Intact chick retina and retinal-homogenate binding assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Millimolar exposure; exact human receptor subunits or oral effects are not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Nicotinic function was blocked without competing at that toxin-binding site.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Agmatine acts as an antagonist of neuronal nicotinic receptors. · 1990 · https://pubmed.ncbi.nlm.nih.gov/2331571/ · DOI 10.1111/j.1476-5381.1990.tb14680.x","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4b2d6d8f-9ec3-57d0-977d-0a18d7c2e429","evidence_kind":"source_excerpt","locator":"Lines 324-330","start_line":324,"end_line":330,"excerpt":"## agmatine-sulfate-nicotinic-chick\nNicotinic function was blocked without competing at that toxin-binding site.\nAgmatine at 1 mM reduced DMPP-induced chick retinal depolarization by approximately 70% without preventing neuronal bungarotoxin binding.\nModel: Intact chick retina and retinal-homogenate binding assays.\nLimitations: Millimolar exposure; exact human receptor subunits or oral effects are not established.\nEvidence access: Primary abstract\nAgmatine acts as an antagonist of neuronal nicotinic receptors. · 1990 · https://pubmed.ncbi.nlm.nih.gov/2331571/ · DOI 10.1111/j.1476-5381.1990.tb14680.x","model_system":"Intact chick retina and retinal-homogenate binding assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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