{"id":"e7fd9e62-bd6d-5fa2-a926-1890fc6fa21d","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-microglia-ap1","predicate":"reduces","statement":"Luteolin reduced AP-1 DNA binding and JNK phosphorylation in LPS-stimulated mouse BV-2 microglia.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ced67ff7-9a74-5459-a616-cef55b0ee986","mechanism_event_label":"One inflammatory signaling route was suppressed.","subject":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"object":{"id":"ce3a8528-797b-559f-aa3c-964946e549cc","slug":"mouse-microglial-ap1-activity","display_name":"Mouse microglial AP-1 DNA-binding activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ced67ff7-9a74-5459-a616-cef55b0ee986","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-microglia-ap1-event","event_type":"observed_relationship","label":"One inflammatory signaling route was suppressed.","description":"Luteolin reduced AP-1 DNA binding and JNK phosphorylation in LPS-stimulated mouse BV-2 microglia.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ce3a8528-797b-559f-aa3c-964946e549cc","slug":"mouse-microglial-ap1-activity","display_name":"Mouse microglial AP-1 DNA-binding activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"556ab115-21d6-55c5-97cd-adc37f22d371","slug":"mouse-jnk-assay-pool","display_name":"Mouse JNK assay pool, isoform unresolved","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse BV-2 and primary-microglia experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pathway readouts do not prove direct JNK binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"One inflammatory signaling route was suppressed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Luteolin reduces IL-6 production in microglia by inhibiting JNK phosphorylation and activation of AP-1. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18490655/ · DOI 10.1073/pnas.0802865105","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"00644227-8fdd-5351-96d8-3d311c89416c","evidence_kind":"source_excerpt","locator":"Lines 404-410","start_line":404,"end_line":410,"excerpt":"## luteolin-microglia-ap1\nOne inflammatory signaling route was suppressed.\nLuteolin reduced AP-1 DNA binding and JNK phosphorylation in LPS-stimulated mouse BV-2 microglia.\nModel: Mouse BV-2 and primary-microglia experiments.\nLimitations: Pathway readouts do not prove direct JNK binding.\nEvidence access: Primary abstract\nLuteolin reduces IL-6 production in microglia by inhibiting JNK phosphorylation and activation of AP-1. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18490655/ · DOI 10.1073/pnas.0802865105","model_system":"Mouse BV-2 and primary-microglia experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"fd6ea0b1cef8d1c9965270494bfa815de14f679044f0b5d5d7bbf4904630ad76","revision_id":"c9a3dd80-c7e6-5fb4-bc91-8db265e7c8de","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}