{"id":"e7e8040f-092d-59e7-b31e-4b095d7019e4","stable_key":"mcm5um34-supports-stress-corrected","predicate":"supports_translation_of","statement":"The correction assigns mcm5Um34 to the stress-related selenoprotein set.","claim_class":"correction","status":"source_derived_draft","evidence_grade":"supplied_source_only","direction":"positive","is_public":true,"mechanism_event_id":null,"mechanism_event_label":null,"subject":{"id":"028e853c-7b1d-5be4-acdd-1c6a84a81b98","slug":"mcm5um34","display_name":"mcm5Um34","entity_type_key":"rna_modification"},"object":{"id":"78d0acd3-415d-5e57-8008-c2f3c43a4d45","slug":"stress-related-selenoproteins","display_name":"Stress-related set","entity_type_key":"protein_set"},"evidence_count":1,"mechanism_event":null,"contexts":[],"evidence":[{"id":"f222d821-eefd-5475-a7c4-025208852c29","evidence_kind":"source_passage","locator":"lines 1-8","start_line":1,"end_line":8,"excerpt":"# Correction first\n\nIn my last message I labeled the tRNA isoforms backwards. Fixing it, because this one matters:\n\n- **mcm⁵U34** (no ribose methyl) → supports **housekeeping** selenoproteins: TXNRD1, GPX4\n- **mcm⁵Um34** (ribose methylated) → **required** for the **stress-related** set: GPX1, SELENOW\n\nThe hierarchy table was right. The labels on the two isoforms were flipped. Um34 is the *added* mark, and it's the one you lose first — which is why the proteins that depend on it die first.","model_system":"Source draft; model details require primary-source verification","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Explicit correction of mcm5U34 and mcm5Um34 assignments.","relationship":"supports","weight":1.0,"link_notes":"Passage support from supplied draft; not yet primary-source verified.","source":{"id":"6b10bfa1-ddd0-52b1-b23e-4c02162b94ab","stable_key":"corrections","title":"Selenium corrections and deep dive","document_type":"user_supplied_markdown","citation_label":"Selenium correction and deep-dive draft","file_path":"X:\\metabolic-ledger\\source_material\\selenium-corrections-and-deep-dive.md","sha256":"ae0bf24b7d9e8b4c7cbc46ac00d84922f467530c1288def216c28640df9e0d00","revision_id":"4cec6304-35d7-5560-9133-e6ea689db838","review_status":"unverified_draft","notes":"Retained as supplied source material. Primary literature verification is still required."}}],"relations":[{"id":"2d976d20-656c-54b1-9256-97e578d366e9","relation_type":"supersedes","notes":"Explicit correction recorded in the supplied correction document.","source_claim_id":"e7e8040f-092d-59e7-b31e-4b095d7019e4","source_claim_key":"mcm5um34-supports-stress-corrected","source_statement":"The correction assigns mcm5Um34 to the stress-related selenoprotein set.","target_claim_id":"02da9a25-f443-513e-bfcc-654d8b4d26f6","target_claim_key":"mcm5um34-supports-housekeeping-reversed","target_statement":"The original draft labeled mcm5Um34 as the housekeeping form."}],"conflicts":[],"corrections":[{"id":"c6f5fc60-d928-581c-87d0-15e0abf2889f","title":"mcm5u34: corrected tRNA isoform labels","kind":"label_reversal","status":"corrected","why":"The earlier draft reversed the two tRNA isoform labels. The author explicitly corrected that error. This is correction history, not an unresolved scientific disagreement.","resolution":"The reversed assignment is superseded. Both source versions remain available in correction history. The later literature review qualifies the replacement wording: modification dependence varies by selenoprotein and experimental context; the two isoforms do not establish a universal housekeeping-versus-stress routing rule.","created_at":"2026-09-17 05:16:58","record_type":"correction","display_label":"Correction history","record_url":"/corrections/c6f5fc60-d928-581c-87d0-15e0abf2889f","literature_review":{"revision_id":"80984e03-5f0f-5877-8094-afef7637444e","start_line":1525,"end_line":1531,"papers":[{"paper_key":"carlson-2005","title":"Selective rescue of selenoprotein expression in mice lacking a highly specialized methyl group in selenocysteine tRNA","url":"https://digitalcommons.unl.edu/biochemgladyshev/54/","doi":"10.1074/jbc.M411725200","year":2005,"model":"Transgenic mouse tRNA replacement; mutant affects i6A37 as well as Um34","summary":"Selenoproteins differ in modification dependence; no universal two-class routing rule."},{"paper_key":"nease-2024","title":"Selenocysteine tRNA methylation promotes oxidative stress resistance in melanoma metastasis","url":"https://www.nature.com/articles/s43018-024-00844-8","doi":"10.1038/s43018-024-00844-8","year":2024,"model":"Biochemistry, human melanoma cells and mouse metastasis/xenograft models","summary":"Identifies FTSJ1 as the Sec-tRNA Um34 methyltransferase."}]},"sides":[{"conflict_id":"c6f5fc60-d928-581c-87d0-15e0abf2889f","ordinal":0,"label":"Original assignment","revision_id":"1fa1ebde-141f-5d47-8a4f-ba87ff84a6c6","start_line":30,"end_line":30,"quote":"- **mcm⁵U** — the \"stress\" form","source_key":"molecular","source_title":"Selenium: the molecular cascade","claim_ids":["8d435309-f139-5602-9f77-6a5c5beacea0"]},{"conflict_id":"c6f5fc60-d928-581c-87d0-15e0abf2889f","ordinal":1,"label":"Explicit source correction","revision_id":"4cec6304-35d7-5560-9133-e6ea689db838","start_line":5,"end_line":5,"quote":"- **mcm⁵U34** (no ribose methyl) → supports **housekeeping** selenoproteins: TXNRD1, GPX4","source_key":"corrections","source_title":"Selenium corrections and deep dive","claim_ids":["bf75305d-1e89-54bb-a3aa-c7cc6ccfc587"]}]},{"id":"65467447-6d61-57f2-b75f-d3098ddeb1e8","title":"mcm5um34: corrected tRNA isoform labels","kind":"label_reversal","status":"corrected","why":"The earlier draft reversed the two tRNA isoform labels. The author explicitly corrected that error. This is correction history, not an unresolved scientific disagreement.","resolution":"The reversed assignment is superseded. Both source versions remain available in correction history. The later literature review qualifies the replacement wording: modification dependence varies by selenoprotein and experimental context; the two isoforms do not establish a universal housekeeping-versus-stress routing rule.","created_at":"2026-09-17 05:16:58","record_type":"correction","display_label":"Correction history","record_url":"/corrections/65467447-6d61-57f2-b75f-d3098ddeb1e8","literature_review":{"revision_id":"80984e03-5f0f-5877-8094-afef7637444e","start_line":1533,"end_line":1539,"papers":[{"paper_key":"carlson-2005","title":"Selective rescue of selenoprotein expression in mice lacking a highly specialized methyl group in selenocysteine tRNA","url":"https://digitalcommons.unl.edu/biochemgladyshev/54/","doi":"10.1074/jbc.M411725200","year":2005,"model":"Transgenic mouse tRNA replacement; mutant affects i6A37 as well as Um34","summary":"Selenoproteins differ in modification dependence; no universal two-class routing rule."},{"paper_key":"nease-2024","title":"Selenocysteine tRNA methylation promotes oxidative stress resistance in melanoma metastasis","url":"https://www.nature.com/articles/s43018-024-00844-8","doi":"10.1038/s43018-024-00844-8","year":2024,"model":"Biochemistry, human melanoma cells and mouse metastasis/xenograft models","summary":"Identifies FTSJ1 as the Sec-tRNA Um34 methyltransferase."}]},"sides":[{"conflict_id":"65467447-6d61-57f2-b75f-d3098ddeb1e8","ordinal":0,"label":"Original assignment","revision_id":"1fa1ebde-141f-5d47-8a4f-ba87ff84a6c6","start_line":31,"end_line":31,"quote":"- **mcm⁵Um** — 2′-O-methylribose added. The \"housekeeping\" form.","source_key":"molecular","source_title":"Selenium: the molecular cascade","claim_ids":["02da9a25-f443-513e-bfcc-654d8b4d26f6"]},{"conflict_id":"65467447-6d61-57f2-b75f-d3098ddeb1e8","ordinal":1,"label":"Explicit source correction","revision_id":"4cec6304-35d7-5560-9133-e6ea689db838","start_line":6,"end_line":6,"quote":"- **mcm⁵Um34** (ribose methylated) → **required** for the **stress-related** set: GPX1, SELENOW","source_key":"corrections","source_title":"Selenium corrections and deep dive","claim_ids":["e7e8040f-092d-59e7-b31e-4b095d7019e4"]}]}],"research":null}