{"id":"e77bfafe-d52b-5749-a4fc-4aa720223caf","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-pdh-activity","predicate":"increases_activity","statement":"PDH activity increased in assays using mitochondrial protein from mangiferin-treated C2C12 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"761f8dea-050f-54fa-bd2b-8b2c4696da87","mechanism_event_label":"An enzyme complex linking glycolysis to mitochondrial metabolism became more active.","subject":{"id":"2cfc0bd9-b5cc-58ab-852a-7705f3d8d47f","slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"},"object":{"id":"a53bcb40-1c97-5845-a48f-6ae54b9867ac","slug":"mouse-pdh-complex","display_name":"Mouse pyruvate dehydrogenase complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"761f8dea-050f-54fa-bd2b-8b2c4696da87","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-pdh-activity-event","event_type":"biochemical_relationship","label":"An enzyme complex linking glycolysis to mitochondrial metabolism became more active.","description":"PDH activity increased in assays using mitochondrial protein from mangiferin-treated C2C12 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"91e6d4f5-fba1-542d-b68a-57cc28e1e425","slug":"pyruvate","display_name":"Pyruvate","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2cfc0bd9-b5cc-58ab-852a-7705f3d8d47f","slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a53bcb40-1c97-5845-a48f-6ae54b9867ac","slug":"mouse-pdh-complex","display_name":"Mouse pyruvate dehydrogenase complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/mangiferin-research/24848064.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5f12809489e236c3dd9888aae3746eb22ff9cbea4271c9f60764bed3b5f9d251\", \"start_char\": 0, \"end_char\": 1761, \"text_sha256\": \"5f12809489e236c3dd9888aae3746eb22ff9cbea4271c9f60764bed3b5f9d251\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"High-fat-fed mice and differentiated C2C12 myotube experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Dietary mangiferin in mice; pharmacological cell exposures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Preclinical experiments; no proof of direct binding or correction of human vitamin deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Mangiferin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An enzyme complex linking glycolysis to mitochondrial metabolism became more active.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mangiferin-p24848064] Mangiferin stimulates carbohydrate oxidation and protects against metabolic disorders induced by high-fat diets. (2014). https://pubmed.ncbi.nlm.nih.gov/24848064/ DOI: 10.2337/db14-0006","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Skeletal muscle and cultured myotubes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"094e2d41-42e6-5238-8996-0b9806164784","evidence_kind":"source_excerpt","locator":"Lines 340-351","start_line":340,"end_line":351,"excerpt":"### mangiferin-pdh-activity\nPDH activity increased in assays using mitochondrial protein from mangiferin-treated C2C12 cells.\nCondition category: normal\nnutrient_topic: Mangiferin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: An enzyme complex linking glycolysis to mitochondrial metabolism became more active.\norganism: Mus musculus\ntissue_or_cell_type: Skeletal muscle and cultured myotubes\nexperimental_model: High-fat-fed mice and differentiated C2C12 myotube experiments\nlimitations: Preclinical experiments; no proof of direct binding or correction of human vitamin deficiency.\nexposure: Dietary mangiferin in mice; pharmacological cell exposures\nevidence_span: {\"source_cache\": \"artifacts/mangiferin-research/24848064.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"5f12809489e236c3dd9888aae3746eb22ff9cbea4271c9f60764bed3b5f9d251\", \"start_char\": 0, \"end_char\": 1761, \"text_sha256\": \"5f12809489e236c3dd9888aae3746eb22ff9cbea4271c9f60764bed3b5f9d251\"}\n[mangiferin-p24848064] Mangiferin stimulates carbohydrate oxidation and protects against metabolic disorders induced by high-fat diets. 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