{"id":"e75498b4-f50c-5d7d-9255-927f4354e820","stable_key":"fcc4fa9c-4dc8-5566-b780-1a486c4d3e72:alcohol-zinc-tight-junctions","predicate":"is_required_in_assay_for","statement":"Zinc deprivation caused epithelial barrier disruption in association with disassembly of tight junction proteins in Caco-2 monolayers, and minor zinc deprivation exaggerated the deleterious effect of alcohol on the epithelial barrier.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"c515d356-dfc5-5f9d-9d06-27aa2251c0f1","mechanism_event_label":"Take zinc away and the seals between cells come apart, with or without alcohol.","subject":{"id":"0429f0ff-c033-514b-9a4f-6f0f83268095","slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"},"object":{"id":"fb05f196-42b6-5fd2-83df-07c2250650d7","slug":"ileal-tight-junctions","display_name":"Tight junction proteins at the ileal epithelium","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c515d356-dfc5-5f9d-9d06-27aa2251c0f1","stable_key":"fcc4fa9c-4dc8-5566-b780-1a486c4d3e72:alcohol-zinc-tight-junctions-event","event_type":"biochemical_relationship","label":"Take zinc away and the seals between cells come apart, with or without alcohol.","description":"Zinc deprivation caused epithelial barrier disruption in association with disassembly of tight junction proteins in Caco-2 monolayers, and minor zinc deprivation exaggerated the deleterious effect of alcohol on the epithelial barrier.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0123b299-b2d4-5c71-b677-7c4364d17af1","slug":"tjp1","display_name":"Human tight-junction protein ZO-1 / TJP1","entity_type_key":"protein"},"role":"tight_junction_protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e653a47d-0723-5479-9572-6ed51aa8d5b0","slug":"ethanol","display_name":"Ethanol","entity_type_key":"drug"},"role":"co_exposure","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0429f0ff-c033-514b-9a4f-6f0f83268095","slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"fb05f196-42b6-5fd2-83df-07c2250650d7","slug":"ileal-tight-junctions","display_name":"Tight junction proteins at the ileal epithelium","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/alcohol-research/20167873.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"12b4bf025e28831a9d613fd7d6b62275f573f8a8999efb73d763bb8e8059b15e\", \"start_char\": 0, \"end_char\": 1542, \"text_sha256\": \"12b4bf025e28831a9d613fd7d6b62275f573f8a8999efb73d763bb8e8059b15e\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Pair-fed alcohol mice with regional permeability measurement and Caco-2 zinc deprivation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Four weeks of alcohol liquid diet; zinc deprivation in culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The regional specificity and the separate zinc-deprivation arm are what make the nutrient claim interpretable. The exaggeration experiment is in cultured cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Alcohol research collection; topical membership is not evidence of a direct clinical effect, and ethanol is recorded separately from the acetaldehyde it becomes.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ethanol","display_name":"Ethanol","entity_type_key":"drug"}},{"dimension":"organism","value_text":"Mouse and human cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Take zinc away and the seals between cells come apart, with or without alcohol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[alcohol-p20167873] The role of zinc deficiency in alcohol-induced intestinal barrier dysfunction. (2010). https://pubmed.ncbi.nlm.nih.gov/20167873/ DOI: 10.1152/ajpgi.00350.2009","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Ileum and Caco-2 monolayers","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"a934d2a7-f564-5af3-8003-1a51815c0007","evidence_kind":"source_excerpt","locator":"Lines 774-785","start_line":774,"end_line":785,"excerpt":"### alcohol-zinc-tight-junctions\nZinc deprivation caused epithelial barrier disruption in association with disassembly of tight junction proteins in Caco-2 monolayers, and minor zinc deprivation exaggerated the deleterious effect of alcohol on the epithelial barrier.\nCondition category: nutrient_deficiency\nnutrient_topic: Alcohol research collection; topical membership is not evidence of a direct clinical effect, and ethanol is recorded separately from the acetaldehyde it becomes.\nplain_language: Take zinc away and the seals between cells come apart, with or without alcohol.\norganism: Mouse and human cells\ntissue_or_cell_type: Ileum and Caco-2 monolayers\nexperimental_model: Pair-fed alcohol mice with regional permeability measurement and Caco-2 zinc deprivation\nlimitations: The regional specificity and the separate zinc-deprivation arm are what make the nutrient claim interpretable. The exaggeration experiment is in cultured cells.\nexposure: Four weeks of alcohol liquid diet; zinc deprivation in culture\nevidence_span: {\"source_cache\": \"artifacts/alcohol-research/20167873.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"12b4bf025e28831a9d613fd7d6b62275f573f8a8999efb73d763bb8e8059b15e\", \"start_char\": 0, \"end_char\": 1542, \"text_sha256\": \"12b4bf025e28831a9d613fd7d6b62275f573f8a8999efb73d763bb8e8059b15e\"}\n[alcohol-p20167873] The role of zinc deficiency in alcohol-induced intestinal barrier dysfunction. (2010). https://pubmed.ncbi.nlm.nih.gov/20167873/ DOI: 10.1152/ajpgi.00350.2009","model_system":"Pair-fed alcohol mice with regional permeability measurement and Caco-2 zinc deprivation","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [alcohol-p20167873] The role of zinc deficiency in alcohol-induced intestinal barrier dysfunction. (2010). https://pubmed.ncbi.nlm.nih.gov/20167873/ DOI: 10.1152/ajpgi.00350.2009","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4b310db0-1387-548c-9e3d-6d2ed634b2cd","stable_key":"import-fcc4fa9c-4dc8-5566-b780-1a486c4d3e72","title":"Alcohol: ethanol clearance, acetaldehyde, the channels it binds, organ injury and nutrient collisions (2026-09-21)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"cb7e8dc390e8bf302cd5e230cadff6ce9847b4aad8a3691a308964d93ba59a85","revision_id":"4b344e1e-4129-579c-a058-50262b03f9a8","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}