{"id":"e73c4bf9-cc81-5ea4-b837-d68044b659be","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-copper-atp","predicate":"reduces","statement":"With 0.5 mM CuSO4 and 10 micrograms/mL myricetin, ATP content fell to 43% of untreated control.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"df4e44b2-60bc-5a82-9b43-23b4609a970b","mechanism_event_label":"Copper changes the outcome of this redox-active compound.","subject":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"object":{"id":"db52e07f-7c1e-57cb-ad66-282e8b3e5fb9","slug":"human-shsy5y-copper-atp","display_name":"Human SH-SY5Y ATP content under copper exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"df4e44b2-60bc-5a82-9b43-23b4609a970b","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-copper-atp-event","event_type":"observed_relationship","label":"Copper changes the outcome of this redox-active compound.","description":"With 0.5 mM CuSO4 and 10 micrograms/mL myricetin, ATP content fell to 43% of untreated control.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"db52e07f-7c1e-57cb-ad66-282e8b3e5fb9","slug":"human-shsy5y-copper-atp","display_name":"Human SH-SY5Y ATP content under copper exposure","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human SH-SY5Y cells, 24 hours; 10 micrograms/mL myricetin is about 31.4 micromolar.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High copper cell-culture challenge; not evidence that ordinary copper foods plus myricetin cause neuronal injury.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Copper changes the outcome of this redox-active compound.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Neurotoxic Effect of Flavonol Myricetin in the Presence of Excess Copper. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33562817/ · DOI 10.3390/molecules26040845","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b9f4722b-48b9-55ff-93d1-79a17f7a456f","evidence_kind":"source_excerpt","locator":"Lines 340-346","start_line":340,"end_line":346,"excerpt":"## myricetin-copper-atp\nCopper changes the outcome of this redox-active compound.\nWith 0.5 mM CuSO4 and 10 micrograms/mL myricetin, ATP content fell to 43% of untreated control.\nModel: Human SH-SY5Y cells, 24 hours; 10 micrograms/mL myricetin is about 31.4 micromolar.\nLimitations: High copper cell-culture challenge; not evidence that ordinary copper foods plus myricetin cause neuronal injury.\nEvidence access: Primary full text\nNeurotoxic Effect of Flavonol Myricetin in the Presence of Excess Copper. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33562817/ · DOI 10.3390/molecules26040845","model_system":"Human SH-SY5Y cells, 24 hours; 10 micrograms/mL myricetin is about 31.4 micromolar.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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