{"id":"e71f8694-6cf4-5119-9a8d-3d44e27eef32","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-rfvt3-silencing","predicate":"supports","statement":"RFVT3-directed siRNA decreased apical radiolabeled-riboflavin uptake by human T84 epithelial cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"e6b88a69-84c7-5215-b875-2a6102064628","mechanism_event_label":"Reducing RFVT3 lowers intestinal-cell riboflavin uptake.","subject":{"id":"036b3828-9ad7-5a41-9e89-119cef5c3665","slug":"slc52a3","display_name":"Riboflavin transporter 3 / SLC52A3","entity_type_key":"protein"},"object":{"id":"8926ef8b-dbce-551f-9f14-160a318bb2eb","slug":"intestinal-riboflavin-uptake","display_name":"Intestinal riboflavin uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e6b88a69-84c7-5215-b875-2a6102064628","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-rfvt3-silencing-event","event_type":"biochemical_relationship","label":"Reducing RFVT3 lowers intestinal-cell riboflavin uptake.","description":"RFVT3-directed siRNA decreased apical radiolabeled-riboflavin uptake by human T84 epithelial cells.","status":"provisional","compartment":{"slug":"plasma-membrane","display_name":"Plasma membrane"},"participants":[{"entity":{"id":"036b3828-9ad7-5a41-9e89-119cef5c3665","slug":"slc52a3","display_name":"Riboflavin transporter 3 / SLC52A3","entity_type_key":"protein"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8926ef8b-dbce-551f-9f14-160a318bb2eb","slug":"intestinal-riboflavin-uptake","display_name":"Intestinal riboflavin uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_location","value_text":"RFVT3 siRNA experiment","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"T84 siRNA and apical tracer-uptake assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"RFVT3-specific siRNA compared with control siRNA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Partial gene silencing in cultured cells; other uptake routes remain possible.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Reducing RFVT3 lowers intestinal-cell riboflavin uptake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[transport-rfvt3-2014] Functional involvement of RFVT3/SLC52A3 in intestinal riboflavin absorption. (2014). https://doi.org/10.1152/ajpgi.00349.2013 DOI: 10.1152/ajpgi.00349.2013","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestinal epithelial T84 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"2215823b-3db2-5664-9f87-ce562f5c3911","evidence_kind":"source_excerpt","locator":"Lines 215-226","start_line":215,"end_line":226,"excerpt":"### transport-rfvt3-silencing\nRFVT3-directed siRNA decreased apical radiolabeled-riboflavin uptake by human T84 epithelial cells.\nCondition category: machinery_impairment\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Reducing RFVT3 lowers intestinal-cell riboflavin uptake.\norganism: Homo sapiens\ntissue_or_cell_type: Intestinal epithelial T84 cells\nexperimental_model: T84 siRNA and apical tracer-uptake assay\nlimitations: Partial gene silencing in cultured cells; other uptake routes remain possible.\nexposure: RFVT3-specific siRNA compared with control siRNA.\nevidence_location: RFVT3 siRNA experiment\n[transport-rfvt3-2014] Functional involvement of RFVT3/SLC52A3 in intestinal riboflavin absorption. (2014). https://doi.org/10.1152/ajpgi.00349.2013 DOI: 10.1152/ajpgi.00349.2013","model_system":"T84 siRNA and apical tracer-uptake assay","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [transport-rfvt3-2014] Functional involvement of RFVT3/SLC52A3 in intestinal riboflavin absorption. (2014). https://doi.org/10.1152/ajpgi.00349.2013 DOI: 10.1152/ajpgi.00349.2013","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"680cb6bc8249877f2410f551807f10d390fdd719014137d7414ba3420e29228d","revision_id":"7a61e299-908d-5372-860b-99ed190f9d7a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}