{"id":"e71a3f21-e1f8-57a6-9d42-379ce940e3f6","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-hif-without-oxidation","predicate":"modulates","statement":"Butyrate still stabilized HIF in human epithelial-cell experiments when its beta-oxidation and associated oxygen consumption were inhibited.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c4149794-b054-560f-9c82-978b9f3f9689","mechanism_event_label":"Fuel burning was not the only way it influenced HIF.","subject":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"object":{"id":"32fa1079-7b37-5178-8fbb-ae1597ea1198","slug":"human-butyrate-hif-stabilization","display_name":"HIF stabilization in butyrate-exposed human epithelial cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c4149794-b054-560f-9c82-978b9f3f9689","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-hif-without-oxidation-event","event_type":"observed_relationship","label":"Fuel burning was not the only way it influenced HIF.","description":"Butyrate still stabilized HIF in human epithelial-cell experiments when its beta-oxidation and associated oxygen consumption were inhibited.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"32fa1079-7b37-5178-8fbb-ae1597ea1198","slug":"human-butyrate-hif-stabilization","display_name":"HIF stabilization in butyrate-exposed human epithelial cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8681acd7-4f56-59e0-a757-37b8376c8bde","slug":"egln1","display_name":"EGLN1 / PHD2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"22b3e241-94b0-55fd-a233-c63af92520d8","slug":"hif1a","display_name":"HIF-1 alpha","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cell experiments with beta-oxidation inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pharmacological inhibition has limitations; the complementary purified-PHD2 assay supports a separate mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Fuel burning was not the only way it influenced HIF.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Microbiota-derived butyrate is an endogenous HIF prolyl hydroxylase inhibitor. · 2021 · https://pubmed.ncbi.nlm.nih.gov/34190032/ · DOI 10.1080/19490976.2021.1938380","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"701876b5-f401-549c-bc8b-173590ff595a","evidence_kind":"source_excerpt","locator":"Lines 254-260","start_line":254,"end_line":260,"excerpt":"## butyrate-hif-without-oxidation\nFuel burning was not the only way it influenced HIF.\nButyrate still stabilized HIF in human epithelial-cell experiments when its beta-oxidation and associated oxygen consumption were inhibited.\nModel: Human cell experiments with beta-oxidation inhibition.\nLimitations: Pharmacological inhibition has limitations; the complementary purified-PHD2 assay supports a separate mechanism.\nEvidence access: Primary abstract\nMicrobiota-derived butyrate is an endogenous HIF prolyl hydroxylase inhibitor. · 2021 · https://pubmed.ncbi.nlm.nih.gov/34190032/ · DOI 10.1080/19490976.2021.1938380","model_system":"Human cell experiments with beta-oxidation inhibition.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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