{"id":"e6c4572a-93e7-5ce7-965e-3badd7e9ae5a","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-osteoclast-ctsk","predicate":"reduces_transcript","statement":"The silicon preparation lowered Cathepsin K mRNA at 48 and 72 hours.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0cb34718-d302-502d-b8d1-7a33d3cfdc20","mechanism_event_label":"A specific osteoclast marker changed during differentiation.","subject":{"id":"1ff7d527-b221-5c48-b48d-a015f7aebe6e","slug":"osa-enriched-silicon-osteoclast-preparation","display_name":"Silicon preparation containing 75% orthosilicic acid","entity_type_key":"chemical_species"},"object":{"id":"74c1b25f-08ad-5fd4-b0af-1302418ceec2","slug":"human-ctsk","display_name":"Human Cathepsin K","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"0cb34718-d302-502d-b8d1-7a33d3cfdc20","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-osteoclast-ctsk-event","event_type":"observed_relationship","label":"A specific osteoclast marker changed during differentiation.","description":"The silicon preparation lowered Cathepsin K mRNA at 48 and 72 hours.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1ff7d527-b221-5c48-b48d-a015f7aebe6e","slug":"osa-enriched-silicon-osteoclast-preparation","display_name":"Silicon preparation containing 75% orthosilicic acid","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"74c1b25f-08ad-5fd4-b0af-1302418ceec2","slug":"human-ctsk","display_name":"Human Cathepsin K","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6db3cbef-401d-5ef3-ab6f-e6949aab7b40","slug":"human-osa-osteoclast-differentiation","display_name":"Human osteoclast differentiation under silicon exposure","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human RANKL-stimulated CD14+ cultures, 50 micrograms Si/mL.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transcript abundance is not direct inhibition of the protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A specific osteoclast marker changed during differentiation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Orthosilicic acid inhibits human osteoclast differentiation and bone resorption. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39405281/ · DOI 10.1371/journal.pone.0312169","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"433df788-b6e2-577e-8871-e4023e4b2b53","evidence_kind":"source_excerpt","locator":"Lines 224-230","start_line":224,"end_line":230,"excerpt":"## silica-osteoclast-ctsk\nA specific osteoclast marker changed during differentiation.\nThe silicon preparation lowered Cathepsin K mRNA at 48 and 72 hours.\nModel: Human RANKL-stimulated CD14+ cultures, 50 micrograms Si/mL.\nLimitations: Transcript abundance is not direct inhibition of the protein.\nEvidence access: Primary full text\nOrthosilicic acid inhibits human osteoclast differentiation and bone resorption. · 2024 · https://pubmed.ncbi.nlm.nih.gov/39405281/ · DOI 10.1371/journal.pone.0312169","model_system":"Human RANKL-stimulated CD14+ cultures, 50 micrograms Si/mL.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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