{"id":"e6411783-d676-56e7-98cc-cace06f4de34","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-nqo2-loss","predicate":"knockdown_increases","statement":"NQO2 RNAi in human K562 cells increased quinone resistance and antioxidant/detoxification enzyme expression, resembling resveratrol treatment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fc66e842-fa27-52fe-8938-71fad1c723bc","mechanism_event_label":"Genetic reduction of a shared melatonin target reproduced part of the response.","subject":{"id":"1c48fce8-58ed-5238-bdfc-6ce3924f20a7","slug":"nqo2","display_name":"Human quinone reductase 2 / NQO2","entity_type_key":"protein"},"object":{"id":"e84ddc83-c5ba-521f-96a3-d9467ade761b","slug":"human-k562-quinone-resistance","display_name":"Quinone resistance in human K562 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fc66e842-fa27-52fe-8938-71fad1c723bc","stable_key":"52871722-4aa6-5453-a902-3e76356db327:resveratrol-nqo2-loss-event","event_type":"observed_relationship","label":"Genetic reduction of a shared melatonin target reproduced part of the response.","description":"NQO2 RNAi in human K562 cells increased quinone resistance and antioxidant/detoxification enzyme expression, resembling resveratrol treatment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1c48fce8-58ed-5238-bdfc-6ce3924f20a7","slug":"nqo2","display_name":"Human quinone reductase 2 / NQO2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e84ddc83-c5ba-521f-96a3-d9467ade761b","slug":"human-k562-quinone-resistance","display_name":"Quinone resistance in human K562 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"53549e32-e6b4-5f09-94e6-62867b015713","slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human leukemia cell culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Phenocopy does not establish that all resveratrol effects are mediated by NQO2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Resveratrol collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"resveratrol","display_name":"Resveratrol","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Genetic reduction of a shared melatonin target reproduced part of the response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Crystal structure of quinone reductase 2 in complex with resveratrol. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15350128/ · DOI 10.1021/bi049162o","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"fb472e85-9206-599a-bcbd-da2379a5153a","evidence_kind":"source_excerpt","locator":"Lines 118-124","start_line":118,"end_line":124,"excerpt":"## resveratrol-nqo2-loss\nGenetic reduction of a shared melatonin target reproduced part of the response.\nNQO2 RNAi in human K562 cells increased quinone resistance and antioxidant/detoxification enzyme expression, resembling resveratrol treatment.\nModel: Human leukemia cell culture.\nLimitations: Phenocopy does not establish that all resveratrol effects are mediated by NQO2.\nEvidence access: Primary abstract\nCrystal structure of quinone reductase 2 in complex with resveratrol. · 2004 · https://pubmed.ncbi.nlm.nih.gov/15350128/ · DOI 10.1021/bi049162o","model_system":"Human leukemia cell culture.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2844eda9-a122-5357-ae47-cd58227c5994","stable_key":"import-52871722-4aa6-5453-a902-3e76356db327","title":"Resveratrol: metabolites, target selectivity and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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