{"id":"e5e7881a-ef8e-5100-9d46-8f41954bdca3","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:mmaa-prefers-apo-mmut-complex","predicate":"forms-cofactor-state-dependent-complex-with","statement":"Human MMAA formed a stable complex preferentially with apo-MMUT and with GMPPNP rather than GDP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"481478f5-88f8-5675-922e-eabd0798c07e","mechanism_event_label":"The purified human proteins assembled differently depending on MMUT cofactor occupancy and nucleotide state.","subject":{"id":"39366dce-a30d-5e74-8c86-2bd8ca0bad96","slug":"mmaa","display_name":"Human MMAA GTPase","entity_type_key":"protein"},"object":{"id":"8f690797-c976-5a5e-886e-dc49d1b17f5a","slug":"mmut","display_name":"Human methylmalonyl-CoA mutase / MMUT","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"481478f5-88f8-5675-922e-eabd0798c07e","stable_key":"7fc92b9e-9cbf-556e-8719-6b5244625250:mmaa-prefers-apo-mmut-complex-event","event_type":"biochemical_relationship","label":"The purified human proteins assembled differently depending on MMUT cofactor occupancy and nucleotide state.","description":"Human MMAA formed a stable complex preferentially with apo-MMUT and with GMPPNP rather than GDP.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"9624279a-7bf6-5f6e-bf30-bb5f6a09fcfe","slug":"human-mmaa-mmut-complex","display_name":"Human MMAA-MMUT complex","entity_type_key":"protein_complex"},"role":"complex","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"07657685-74aa-5d99-8625-0f6a3b521f07","slug":"adenosylcobalamin","display_name":"5'-Deoxyadenosylcobalamin","entity_type_key":"small_molecule"},"role":"cofactor-occupancy-variable","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"39366dce-a30d-5e74-8c86-2bd8ca0bad96","slug":"mmaa","display_name":"Human MMAA GTPase","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8f690797-c976-5a5e-886e-dc49d1b17f5a","slug":"mmut","display_name":"Human methylmalonyl-CoA mutase / MMUT","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Indexed primary abstract; indexed Results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human proteins","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Apo versus holo-MMUT; GMPPNP versus GDP","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"GMPPNP is a GTP analogue; stable-complex abundance is not cofactor transfer rate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b12","display_name":"Vitamin B12 (cobalamins)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The purified human proteins assembled differently depending on MMUT cofactor occupancy and nucleotide state.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[froese-2010-human-mmaa] Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation. (2010). https://pubmed.ncbi.nlm.nih.gov/20876572/ DOI: 10.1074/jbc.m110.177717","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified protein assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e637849d-f378-57d5-bde4-a0296b14d6de","evidence_kind":"source_excerpt","locator":"Lines 1047-1059","start_line":1047,"end_line":1059,"excerpt":"### mmaa-prefers-apo-mmut-complex\nHuman MMAA formed a stable complex preferentially with apo-MMUT and with GMPPNP rather than GDP.\nCondition category: normal\nnutrient_topic: Vitamin B12 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The purified human proteins assembled differently depending on MMUT cofactor occupancy and nucleotide state.\norganism: Homo sapiens\ntissue_or_cell_type: Purified protein assay\nexperimental_model: Purified human proteins\nlimitations: GMPPNP is a GTP analogue; stable-complex abundance is not cofactor transfer rate.\nexposure: Apo versus holo-MMUT; GMPPNP versus GDP\ncross_nutrient: false\nevidence_location: Indexed primary abstract; indexed Results\n[froese-2010-human-mmaa] Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation. (2010). https://pubmed.ncbi.nlm.nih.gov/20876572/ DOI: 10.1074/jbc.m110.177717","model_system":"Purified human proteins","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [froese-2010-human-mmaa] Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation. (2010). https://pubmed.ncbi.nlm.nih.gov/20876572/ DOI: 10.1074/jbc.m110.177717","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73145023-2982-5848-aff2-8d8875d6b9c5","stable_key":"import-7fc92b9e-9cbf-556e-8719-6b5244625250","title":"Vitamin B12: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ad5b3a51d36e856aa7fdfd1cc23f690b094689bad87d28e1a621a54675a01c92","revision_id":"118bd616-0c13-549f-bfeb-bc439715b89c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}