{"id":"e5746241-5c76-5992-bfed-81b5559a5540","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:lariciresinol-lowers-heat-shock-protein-27","predicate":"reduces","statement":"Lariciresinol lowered heat shock protein 27 in hepatoma cells, confirmed by western blotting, within a set of eight differentially expressed proteins whose network analysis placed the ubiquitin-conjugating enzyme at the hub.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"4c3d9c11-59de-5869-90f0-f89662315d1f","mechanism_event_label":"Lariciresinol lowered heat shock protein 27 in hepatoma cells, confirmed by western blotting, within a set of eight differentially expressed proteins whose network analysis placed the ubiquitin-conjugating enzyme at the hub.","subject":{"id":"4fcf5405-bafb-54bf-89b8-2fc11e0d9822","slug":"lariciresinol","display_name":"Lariciresinol, enantiomer unresolved","entity_type_key":"small_molecule"},"object":{"id":"36f38a56-0221-5f6c-b617-7c8838dd0e75","slug":"hspb1","display_name":"Heat shock protein 27 / HSPB1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"4c3d9c11-59de-5869-90f0-f89662315d1f","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:lariciresinol-lowers-heat-shock-protein-27-event","event_type":"observed_relationship","label":"Lariciresinol lowered heat shock protein 27 in hepatoma cells, confirmed by western blotting, within a set of eight differentially expressed proteins whose network analysis placed the ubiquitin-conjugating enzyme at the hub.","description":"Lariciresinol lowered heat shock protein 27 in hepatoma cells, confirmed by western blotting, within a set of eight differentially expressed proteins whose network analysis placed the ubiquitin-conjugating enzyme at the hub.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4fcf5405-bafb-54bf-89b8-2fc11e0d9822","slug":"lariciresinol","display_name":"Lariciresinol, enantiomer unresolved","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"36f38a56-0221-5f6c-b617-7c8838dd0e75","slug":"hspb1","display_name":"Heat shock protein 27 / HSPB1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HepG2 hepatoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lariciresinol","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Three proteins rose and five fell; the network hub is a computational inference from the eight identified proteins rather than a measured interaction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human HepG2 hepatoma cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Lariciresinol lowered heat shock protein 27 in hepatoma cells, confirmed by western blotting, within a set of eight differentially expressed proteins whose network analysis placed the ubiquitin-conjugating enzyme at the hub.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Proteomic analysis of apoptosis induction by lariciresinol in human HepG2 cells. (2016). https://pubmed.ncbi.nlm.nih.gov/27417256/ DOI: 10.1016/j.cbi.2016.07.013","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Proteome by two-dimensional electrophoresis with western blot confirmation","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"21d44bc8-3e1e-55bb-a9c8-abded8e7e4bc","evidence_kind":"source_excerpt","locator":"Lines 198-207","start_line":198,"end_line":207,"excerpt":"## lariciresinol-lowers-heat-shock-protein-27\nLariciresinol lowered heat shock protein 27 in hepatoma cells, confirmed by western blotting, within a set of eight differentially expressed proteins whose network analysis placed the ubiquitin-conjugating enzyme at the hub.\nModel/species: Human HepG2 hepatoma cells\nTissue/system: Proteome by two-dimensional electrophoresis with western blot confirmation\nExposure: Lariciresinol\nRoute: In vitro\nDuration: Not stated here\nLimits: Three proteins rose and five fell; the network hub is a computational inference from the eight identified proteins rather than a measured interaction.\nPrimary reference: Proteomic analysis of apoptosis induction by lariciresinol in human HepG2 cells. (2016). https://pubmed.ncbi.nlm.nih.gov/27417256/ DOI: 10.1016/j.cbi.2016.07.013\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"48c58761-0291-5bad-a926-75e904b2d802","stable_key":"import-96d9034a-2550-5f3c-b767-1767c982533c","title":"Lariciresinol: five molecules under one name, and the mechanisms each one carries (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twelve primary studies, every abstract read and all DOIs cross-checked against live PubMed metadata. Mechanism edges only, with no conclusion or claim of benefit recorded. Three author clusters account for eight of the twelve and carry shared laboratory keys. Study-specific concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"6bf0015b7ff727b7a80d693dd18ffdd227d0e16ae45d9e85a5f81fa733edd3a4","revision_id":"219d9b0c-1a71-5e27-8d6c-7b3e77a594c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}