{"id":"e543ed0e-e771-5d8c-8bd6-cfac5ab010aa","stable_key":"c8c7aa13-14c6-5f5c-9afc-9217d0f2f736:hfcs-glut5","predicate":"transports","statement":"Expressed human GLUT5 selectively transported fructose, with a reported Km near 6 mM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"dcde5f2e-4ee5-5e75-956d-ec088c361851","mechanism_event_label":"Expressed human GLUT5 selectively transported fructose, with a reported Km near 6 mM.","subject":{"id":"2ab8fe70-398e-5537-8992-6f692b478258","slug":"slc2a5","display_name":"Human fructose transporter GLUT5 / SLC2A5","entity_type_key":"protein"},"object":{"id":"3c379840-5688-5b5d-84c0-5950b4fd8f17","slug":"fructose","display_name":"Fructose","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"dcde5f2e-4ee5-5e75-956d-ec088c361851","stable_key":"c8c7aa13-14c6-5f5c-9afc-9217d0f2f736:hfcs-glut5-event","event_type":"observed_relationship","label":"Expressed human GLUT5 selectively transported fructose, with a reported Km near 6 mM.","description":"Expressed human GLUT5 selectively transported fructose, with a reported Km near 6 mM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2ab8fe70-398e-5537-8992-6f692b478258","slug":"slc2a5","display_name":"Human fructose transporter GLUT5 / SLC2A5","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3c379840-5688-5b5d-84c0-5950b4fd8f17","slug":"fructose","display_name":"Fructose","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Fructose kinetic series; reported Km about 6 mM","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Assay interval unavailable in abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract/metadata; unrecovered methods explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human GLUT5 expressed in Xenopus oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_scope","value_text":"Isolated fructose / human protein","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human protein in an amphibian expression system; not a measured human intake threshold.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"HFCS chapter: actual formulation studies, component biochemistry and interventions are explicitly distinguished.","comparator":null,"unit":null,"notes":"","entity":{"slug":"high-fructose-corn-syrup","display_name":"High-Fructose Corn Syrup / HFCS","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human GLUT5 expressed in Xenopus oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Expressed human GLUT5 selectively transported fructose, with a reported Km near 6 mM.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Fructose transporter in human spermatozoa and small intestine is GLUT5. (1992). https://pubmed.ncbi.nlm.nih.gov/1634504/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro substrate addition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Recombinant transport assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Fructose uptake measured in the expressing cell with a reported Km near 6 mM.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Fructose uptake measured in the expressing cell with a reported Km near 6 mM.","entity":null}],"evidence":[{"id":"500ebc30-bcd8-52e1-9e27-ac7dfae7a61e","evidence_kind":"source_excerpt","locator":"Lines 65-75","start_line":65,"end_line":75,"excerpt":"## hfcs-glut5\nExpressed human GLUT5 selectively transported fructose, with a reported Km near 6 mM.\nModel/species: Human GLUT5 expressed in Xenopus oocytes\nTissue: Recombinant transport assay\nExposure: Fructose kinetic series; reported Km about 6 mM\nRoute: In vitro substrate addition\nDuration: Assay interval unavailable in abstract\nExposure scope: Isolated fructose / human protein\nLimits: Human protein in an amphibian expression system; not a measured human intake threshold.\nReference: Fructose transporter in human spermatozoa and small intestine is GLUT5. (1992). https://pubmed.ncbi.nlm.nih.gov/1634504/\nAccess: Primary abstract/metadata; unrecovered methods explicitly retained.","model_system":"Human GLUT5 expressed in Xenopus oocytes","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; provenance retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e863ddf6-7c1e-5a70-8335-7f3ac3eeee92","stable_key":"import-c8c7aa13-14c6-5f5c-9afc-9217d0f2f736","title":"High-Fructose Corn Syrup: mechanism of action and metabolic impact (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twenty primary studies and official FDA composition information, with one reused canonical glucose-transport claim. Study-specific citations, negative findings and limitations retained. 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