{"id":"e4f21f2f-a9ba-58bb-bebf-27e483c2f895","stable_key":"4fef8d72-f107-5eb5-b2fb-483ca85465e0:k2-gas6-decoy","predicate":"fails_to_activate","statement":"Uncarboxylated GAS6 and selected domain-deletion variants retained TAM binding but acted as blocking decoy ligands rather than activators.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0878627d-b387-5593-9b78-b3791e32ea62","mechanism_event_label":"The missing modification changed what happened after contact.","subject":{"id":"66558c97-e07d-52be-8f46-bacecc5a7d5c","slug":"uncarboxylated-gas6","display_name":"Uncarboxylated human GAS6","entity_type_key":"protein_state"},"object":{"id":"463b8fd0-660b-5173-bd7a-57485e599031","slug":"tam-receptor-activation","display_name":"TYRO3/AXL/MERTK receptor activation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0878627d-b387-5593-9b78-b3791e32ea62","stable_key":"4fef8d72-f107-5eb5-b2fb-483ca85465e0:k2-gas6-decoy-event","event_type":"biochemical_relationship","label":"The missing modification changed what happened after contact.","description":"Uncarboxylated GAS6 and selected domain-deletion variants retained TAM binding but acted as blocking decoy ligands rather than activators.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"66558c97-e07d-52be-8f46-bacecc5a7d5c","slug":"uncarboxylated-gas6","display_name":"Uncarboxylated human GAS6","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"463b8fd0-660b-5173-bd7a-57485e599031","slug":"tam-receptor-activation","display_name":"TYRO3/AXL/MERTK receptor activation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/k2-research/29176978.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"14dfae68318cd2654ee423539418eb489da66e26117e35473e99986b29b8efd7\", \"start_char\": 0, \"end_char\": 1704, \"text_sha256\": \"14dfae68318cd2654ee423539418eb489da66e26117e35473e99986b29b8efd7\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"TAM reporter cells and GAS6 domain/point mutagenesis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Warfarin, Gla/EGF mutations and PS-positive cells or vesicles","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Receptor binding and receptor activation are different endpoints; no clinical K2 immune benefit was tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin K2 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"k2","display_name":"Vitamin K2 / menaquinone family","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Recombinant human GAS6 and TAM reporter systems","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The missing modification changed what happened after contact.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[k2-p29176978] Requirement of Gamma-Carboxyglutamic Acid Modification and Phosphatidylserine Binding for the Activation of Tyro3, Axl, and Mertk Receptors by Growth Arrest-Specific 6. (2017). https://pubmed.ncbi.nlm.nih.gov/29176978/ DOI: 10.3389/fimmu.2017.01521","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Phosphatidylserine-bearing surfaces and receptors","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"5cb8a6f3-5e31-5efc-8d84-b7b9f8902009","evidence_kind":"source_excerpt","locator":"Lines 617-628","start_line":617,"end_line":628,"excerpt":"### k2-gas6-decoy\nUncarboxylated GAS6 and selected domain-deletion variants retained TAM binding but acted as blocking decoy ligands rather than activators.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin K2 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The missing modification changed what happened after contact.\norganism: Recombinant human GAS6 and TAM reporter systems\ntissue_or_cell_type: Phosphatidylserine-bearing surfaces and receptors\nexperimental_model: TAM reporter cells and GAS6 domain/point mutagenesis\nlimitations: Receptor binding and receptor activation are different endpoints; no clinical K2 immune benefit was tested.\nexposure: Warfarin, Gla/EGF mutations and PS-positive cells or vesicles\nevidence_span: {\"source_cache\": \"artifacts/k2-research/29176978.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"14dfae68318cd2654ee423539418eb489da66e26117e35473e99986b29b8efd7\", \"start_char\": 0, \"end_char\": 1704, \"text_sha256\": \"14dfae68318cd2654ee423539418eb489da66e26117e35473e99986b29b8efd7\"}\n[k2-p29176978] Requirement of Gamma-Carboxyglutamic Acid Modification and Phosphatidylserine Binding for the Activation of Tyro3, Axl, and Mertk Receptors by Growth Arrest-Specific 6. 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