{"id":"e4e94736-7e14-53b3-a344-a03379e18388","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip14-manganese-influx","predicate":"increases","statement":"Mouse ZIP14 directly mediated 54Mn(II) uptake in RNA-injected Xenopus oocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"77a6bf69-346a-585d-b7b7-e8e7ae792f4e","mechanism_event_label":"ZIP14 could carry manganese as well as zinc in this experiment.","subject":{"id":"9e0e70ec-aa5b-584d-a6ea-28c400cb3100","slug":"mouse-slc39a14","display_name":"Mouse ZIP14 (Slc39a14)","entity_type_key":"protein"},"object":{"id":"ef134340-d6ae-5c8f-8b76-303bcc66760a","slug":"cellular-manganese-uptake","display_name":"Cellular manganese uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"77a6bf69-346a-585d-b7b7-e8e7ae792f4e","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-zip14-manganese-influx-event","event_type":"biochemical_relationship","label":"ZIP14 could carry manganese as well as zinc in this experiment.","description":"Mouse ZIP14 directly mediated 54Mn(II) uptake in RNA-injected Xenopus oocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"shared_transporter_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9e0e70ec-aa5b-584d-a6ea-28c400cb3100","slug":"mouse-slc39a14","display_name":"Mouse ZIP14 (Slc39a14)","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ef134340-d6ae-5c8f-8b76-303bcc66760a","slug":"cellular-manganese-uptake","display_name":"Cellular manganese uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"true","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"RNA-injected Xenopus oocytes with radiolabeled metal uptake","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"54Mn transport compared with uninjected controls.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Transport capacity is not proof that manganese supplements improve zinc transport or vice versa.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse protein in Xenopus laevis oocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"ZIP14 could carry manganese as well as zinc in this experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zinc-trans-21653899] Zip14 is a complex broad-scope metal-ion transporter whose functional properties support roles in the cellular uptake of zinc and nontransferrin-bound iron. (2011). https://pubmed.ncbi.nlm.nih.gov/21653899/ DOI: 10.1152/ajpcell.00479.2010","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Oocyte plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f81c2ec4-50b2-580d-93c2-567ea1f2a6a1","evidence_kind":"source_excerpt","locator":"Lines 492-503","start_line":492,"end_line":503,"excerpt":"### zinc-trans-zip14-manganese-influx\nMouse ZIP14 directly mediated 54Mn(II) uptake in RNA-injected Xenopus oocytes.\nCondition category: normal\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: ZIP14 could carry manganese as well as zinc in this experiment.\norganism: Mouse protein in Xenopus laevis oocytes\ntissue_or_cell_type: Oocyte plasma membrane\nexperimental_model: RNA-injected Xenopus oocytes with radiolabeled metal uptake\nlimitations: Transport capacity is not proof that manganese supplements improve zinc transport or vice versa.\nexposure: 54Mn transport compared with uninjected controls.\ncross_nutrient: true\n[zinc-trans-21653899] Zip14 is a complex broad-scope metal-ion transporter whose functional properties support roles in the cellular uptake of zinc and nontransferrin-bound iron. (2011). https://pubmed.ncbi.nlm.nih.gov/21653899/ DOI: 10.1152/ajpcell.00479.2010","model_system":"RNA-injected Xenopus oocytes with radiolabeled metal uptake","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zinc-trans-21653899] Zip14 is a complex broad-scope metal-ion transporter whose functional properties support roles in the cellular uptake of zinc and nontransferrin-bound iron. (2011). https://pubmed.ncbi.nlm.nih.gov/21653899/ DOI: 10.1152/ajpcell.00479.2010","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2e731dd54477ec1254e97df3323e1208d38f1375effed19252e71ab4f600d13a","revision_id":"c72258b9-ac09-5408-9d44-a921ad1f96a3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}