{"id":"e4e56f1a-f1cd-5f80-a507-2c573f6b1e8f","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-s-high-concentration-inhibition","predicate":"can_inhibit_reduction_of","statement":"S-lipoic acid inhibited R-lipoic-acid reduction only at high concentrations in the enzyme assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1eede49c-4667-5c67-b9ae-1505c6492869","mechanism_event_label":"Competition appeared under particular assay conditions, not as proof that ordinary racemic supplements block the pathway.","subject":{"id":"2b62d746-e407-580b-b077-1fc83a923cbb","slug":"s-lipoic-acid","display_name":"Free S-alpha-lipoic acid","entity_type_key":"small_molecule"},"object":{"id":"8eeb8f85-a924-5122-9399-a757b83c668c","slug":"r-lipoic-acid","display_name":"Free R-alpha-lipoic acid","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"1eede49c-4667-5c67-b9ae-1505c6492869","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-s-high-concentration-inhibition-event","event_type":"biochemical_relationship","label":"Competition appeared under particular assay conditions, not as proof that ordinary racemic supplements block the pathway.","description":"S-lipoic acid inhibited R-lipoic-acid reduction only at high concentrations in the enzyme assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"886512d6-6c63-5c64-8639-d443ee9f395b","slug":"mitochondrial-lipoamide-dehydrogenases","display_name":"Mitochondrial lipoamide dehydrogenases, species unspecified","entity_type_key":"protein_family"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2b62d746-e407-580b-b077-1fc83a923cbb","slug":"s-lipoic-acid","display_name":"Free S-alpha-lipoic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8eeb8f85-a924-5122-9399-a757b83c668c","slug":"r-lipoic-acid","display_name":"Free R-alpha-lipoic acid","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ala-research/8573188.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7631d83a60fe421318384db96c5b92458c21b0e3aeadaff85c7ae60223e40976\", \"start_char\": 0, \"end_char\": 809, \"text_sha256\": \"7631d83a60fe421318384db96c5b92458c21b0e3aeadaff85c7ae60223e40976\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified mitochondrial lipoamide dehydrogenase stereoselectivity assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"R/S lipoic acid and metabolites","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not equate the enzyme-family result with a specific human DLD preparation or whole-person clinical superiority.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Species not identified in indexed abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Competition appeared under particular assay conditions, not as proof that ordinary racemic supplements block the pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ala-p8573188] Reduction of lipoic acid by lipoamide dehydrogenase. (1996). https://pubmed.ncbi.nlm.nih.gov/8573188/ DOI: 10.1016/0006-2952(95)02124-8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified mitochondrial enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d2d387a5-1b1d-5352-968a-e4e052e6c7b2","evidence_kind":"source_excerpt","locator":"Lines 780-791","start_line":780,"end_line":791,"excerpt":"### ala-s-high-concentration-inhibition\nS-lipoic acid inhibited R-lipoic-acid reduction only at high concentrations in the enzyme assay.\nCondition category: normal\nnutrient_topic: Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Competition appeared under particular assay conditions, not as proof that ordinary racemic supplements block the pathway.\norganism: Species not identified in indexed abstract\ntissue_or_cell_type: Purified mitochondrial enzyme\nexperimental_model: Purified mitochondrial lipoamide dehydrogenase stereoselectivity assays\nlimitations: Do not equate the enzyme-family result with a specific human DLD preparation or whole-person clinical superiority.\nexposure: R/S lipoic acid and metabolites\nevidence_span: {\"source_cache\": \"artifacts/ala-research/8573188.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"7631d83a60fe421318384db96c5b92458c21b0e3aeadaff85c7ae60223e40976\", \"start_char\": 0, \"end_char\": 809, \"text_sha256\": \"7631d83a60fe421318384db96c5b92458c21b0e3aeadaff85c7ae60223e40976\"}\n[ala-p8573188] Reduction of lipoic acid by lipoamide dehydrogenase. 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