{"id":"e4a60501-227d-5f0b-a9eb-283068e34e73","stable_key":"8600a864-6653-52c7-9718-1362c5af4577:gamma-nonalactone-capsaicin-low","predicate":"sensitizes_capsaicin_response","statement":"Lower gamma-nonalactone concentrations shifted the capsaicin response toward greater sensitivity with little change in maximum response.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"6209d26f-7c04-57ac-a60d-b86b0762dbf1","mechanism_event_label":"Lower gamma-nonalactone concentrations shifted the capsaicin response toward greater sensitivity with little change in maximum response.","subject":{"id":"2b85fa4a-5c91-5de9-aead-a443bc4394af","slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"},"object":{"id":"8e0c61d8-3930-51d5-bce2-25a5edfb797c","slug":"human-trpv1","display_name":"Human transient receptor potential vanilloid 1 / TRPV1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"6209d26f-7c04-57ac-a60d-b86b0762dbf1","stable_key":"8600a864-6653-52c7-9718-1362c5af4577:gamma-nonalactone-capsaicin-low-event","event_type":"observed_relationship","label":"Lower gamma-nonalactone concentrations shifted the capsaicin response toward greater sensitivity with little change in maximum response.","description":"Lower gamma-nonalactone concentrations shifted the capsaicin response toward greater sensitivity with little change in maximum response.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2b85fa4a-5c91-5de9-aead-a443bc4394af","slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8e0c61d8-3930-51d5-bce2-25a5edfb797c","slug":"human-trpv1","display_name":"Human transient receptor potential vanilloid 1 / TRPV1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"dose","value_text":"Gamma-nonalactone 0.64 or 1.28 mM with a capsaicin concentration series","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Acute calcium response","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary full-text methods/results inspected; PubMed metadata where indexed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human TRPV1-expressing HEK293 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Functional curve shifts do not directly locate a binding site. No human pungency or pain outcome was tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Gamma-nonalactone flavor-compound chapter; nutrient and drug interactions retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"gamma-nonalactone","display_name":"Gamma-nonalactone","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human TRPV1-expressing HEK293 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Lower gamma-nonalactone concentrations shifted the capsaicin response toward greater sensitivity with little change in maximum response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Agonistic/antagonistic properties of lactones in food flavors on the sensory ion channels TRPV1 and TRPA1. (2022). https://pubmed.ncbi.nlm.nih.gov/36374622/ DOI: 10.1093/chemse/bjac023","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro co-application","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Capsaicin concentration-response assay","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9904d4e4-1dde-50d3-bd7a-9b19e933ba61","evidence_kind":"source_excerpt","locator":"Lines 37-46","start_line":37,"end_line":46,"excerpt":"## gamma-nonalactone-capsaicin-low\nLower gamma-nonalactone concentrations shifted the capsaicin response toward greater sensitivity with little change in maximum response.\nModel/species: Human TRPV1-expressing HEK293 cells\nTissue: Capsaicin concentration-response assay\nExposure: Gamma-nonalactone 0.64 or 1.28 mM with a capsaicin concentration series\nRoute: In vitro co-application\nDuration: Acute calcium response\nLimits: Functional curve shifts do not directly locate a binding site. No human pungency or pain outcome was tested.\nPrimary reference: Agonistic/antagonistic properties of lactones in food flavors on the sensory ion channels TRPV1 and TRPA1. (2022). https://pubmed.ncbi.nlm.nih.gov/36374622/ DOI: 10.1093/chemse/bjac023\nAccess: Primary full-text methods/results inspected; PubMed metadata where indexed.","model_system":"Human TRPV1-expressing HEK293 cells","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73777c54-95a4-5b07-9d14-d4dd31949fb1","stable_key":"import-8600a864-6653-52c7-9718-1362c5af4577","title":"Gamma-nonalactone: mechanisms, molecular forms and cross-actor connections (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of eight primary studies. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"a6fede6fbb2b70669be6846626f9c197edfd605c627d50fffa984e2b0b7ed71d","revision_id":"bb5ac9d2-d97d-59ac-a99c-ddf46d535574","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}