{"id":"e466dfad-f9c9-504e-b5ae-8548677de976","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-wars1-charging","predicate":"aminoacylates","statement":"Human WARS1 recognizes tryptophan and its cognate tRNA; ATP-dependent activation precedes transfer to tRNA in protein-synthesis aminoacylation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"be31eb58-9f09-5a2d-b631-4b352688918b","mechanism_event_label":"Tryptophan is a building block that must be attached to the correct transfer RNA.","subject":{"id":"d14146bc-16c2-5f9e-98fb-6813f0a0210c","slug":"wars1","display_name":"Human cytosolic tryptophanyl-tRNA synthetase / WARS1","entity_type_key":"protein"},"object":{"id":"33eab802-cc65-51ea-8e61-0a9c5696455c","slug":"human-tryptophanyl-trna","display_name":"Human tryptophanyl-tRNA-Trp","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"be31eb58-9f09-5a2d-b631-4b352688918b","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-wars1-charging-event","event_type":"observed_relationship","label":"Tryptophan is a building block that must be attached to the correct transfer RNA.","description":"Human WARS1 recognizes tryptophan and its cognate tRNA; 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molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Tryptophan is a building block that must be attached to the correct transfer RNA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Structure of human tryptophanyl-tRNA synthetase in complex with tRNATrp reveals the molecular basis of tRNA recognition and specificity. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16798914/ · DOI 10.1093/nar/gkl441","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4a4f9e2d-e555-5cea-8f87-a91de896e3e1","evidence_kind":"source_excerpt","locator":"Lines 114-120","start_line":114,"end_line":120,"excerpt":"## tryptophan-wars1-charging\nTryptophan is a building block that must be attached to the correct transfer RNA.\nHuman WARS1 recognizes tryptophan and its cognate tRNA; ATP-dependent activation precedes transfer to tRNA in protein-synthesis aminoacylation.\nModel: Human WARS1–tRNA structural analysis and biochemical aminoacylation.\nLimitations: Cytosolic WARS1 is distinct from mitochondrial WARS2.\nEvidence access: Primary full text, aminoacylation mechanism and structural results\nStructure of human tryptophanyl-tRNA synthetase in complex with tRNATrp reveals the molecular basis of tRNA recognition and specificity. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16798914/ · DOI 10.1093/nar/gkl441","model_system":"Human WARS1–tRNA structural analysis and biochemical aminoacylation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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