{"id":"e42b75d3-5877-58ad-ac74-9f3f41a2b686","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:antibiotic-metabolism","predicate":"decreases_in_recorded_experiment","statement":"Antibiotic treatment suppressed characteristic shikimate-derived metabolic products in rats.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"b0caa7be-6306-55c1-8f78-88b0ba82cbd6","mechanism_event_label":"Antibiotic treatment suppressed characteristic shikimate-derived metabolic products in rats.","subject":{"id":"67ad4a8f-1bf6-59e5-90a6-97b7709024d3","slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"},"object":{"id":"05e144a6-a15a-5e92-81cc-9972e1b61c7d","slug":"rat-shikimate-microbial-conversion","display_name":"Rat gut-dependent shikimate biotransformation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b0caa7be-6306-55c1-8f78-88b0ba82cbd6","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:antibiotic-metabolism-event","event_type":"experimental_observation","label":"Antibiotic treatment suppressed characteristic shikimate-derived metabolic products in rats.","description":"**Gut metabolism and host conjugation must be distinguished.** The 1978 rat study traced oral shikimic acid into urinary hippurate, hexahydrohippurate, tetrahydrohippurate, two dihydroxycyclohexanecarboxylate isomers, catechol conjugates and expired CO2. Antibiotic treatment suppressed the characteristic conversion, implicating initial gut-microbial transformations. Host metabolism of microbial products still occurs. Hippurate contains a glycine conjugate, but product detection does not establish clinically significant glycine depletion or an identical human quantitative pathway. No assertion that parent shikimic acid is directly converted to every final product in one reaction is made. [The metabolism of shikimate in the rat.](https://pubmed.ncbi.nlm.nih.gov/637841/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67ad4a8f-1bf6-59e5-90a6-97b7709024d3","slug":"shikimic-acid","display_name":"Shikimic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Shikimic acid after antibiotic treatment","sequence_order":0,"notes":""},{"entity":{"id":"05e144a6-a15a-5e92-81cc-9972e1b61c7d","slug":"rat-shikimate-microbial-conversion","display_name":"Rat gut-dependent shikimate biotransformation","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full results, tables and supplements not independently extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Shikimic acid after antibiotic treatment\", \"comparator\": \"Shikimic acid without antibiotic treatment\", \"endpoint\": \"Antibiotic treatment suppressed characteristic shikimate-derived metabolic products in rats.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Rat oral tracer study with antibiotic perturbation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Broad microbial perturbation, not selective EPSPS inhibition or lack of host metabolism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Antibiotic treatment suppressed characteristic shikimate-derived metabolic products in rats.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The metabolism of shikimate in the rat. | 1978 | DOI 10.1042/bj1700257 | PMID 637841 | https://pubmed.ncbi.nlm.nih.gov/637841/ | https://doi.org/10.1042/bj1700257 | https://pmc.ncbi.nlm.nih.gov/articles/PMC1183892/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 85-85; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"5d381edf-00ad-5501-8c3b-bb02e98d99e2","evidence_kind":"source_excerpt","locator":"Lines 85-85","start_line":85,"end_line":85,"excerpt":"**Gut metabolism and host conjugation must be distinguished.** The 1978 rat study traced oral shikimic acid into urinary hippurate, hexahydrohippurate, tetrahydrohippurate, two dihydroxycyclohexanecarboxylate isomers, catechol conjugates and expired CO2. Antibiotic treatment suppressed the characteristic conversion, implicating initial gut-microbial transformations. Host metabolism of microbial products still occurs. Hippurate contains a glycine conjugate, but product detection does not establish clinically significant glycine depletion or an identical human quantitative pathway. No assertion that parent shikimic acid is directly converted to every final product in one reaction is made. [The metabolism of shikimate in the rat.](https://pubmed.ncbi.nlm.nih.gov/637841/)","model_system":"Rat oral tracer study with antibiotic perturbation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e6ae59de-0369-5c2f-8262-57d91302671c","stable_key":"import-31b1baa4-4113-5541-b9e7-fe44a5253a07","title":"Shikimic acid: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"95b1f9e9577661312d67f36e156d2e49326b207f296c1c0e801a5b007fd8e283","revision_id":"cd3237f1-131a-557d-84b5-7543259807b0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}