{"id":"e423259a-9d77-5c79-8beb-d82adf214910","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-gsr-free-lipoate","predicate":"reduces","statement":"Purified human glutathione reductase reduced free lipoic acid to DHLA in vitro.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b8abe820-4f03-5f2f-8b46-e6cf80f28c0b","mechanism_event_label":"An enzyme associated with glutathione recycling can also reduce free lipoic acid.","subject":{"id":"ae04c12f-c0ac-5c8d-9ee2-76e58156d105","slug":"gsr","display_name":"Glutathione reductase / GSR","entity_type_key":"protein"},"object":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"b8abe820-4f03-5f2f-8b46-e6cf80f28c0b","stable_key":"5d8e27d8-6a74-5560-827f-3f90908bbc34:ala-gsr-free-lipoate-event","event_type":"biochemical_relationship","label":"An enzyme associated with glutathione recycling can also reduce free lipoic acid.","description":"Purified human glutathione reductase reduced free lipoic acid to DHLA in vitro.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"440768d2-007b-5a78-b340-889759fbdb05","slug":"dihydrolipoic-acid","display_name":"Free dihydrolipoic acid / DHLA","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"reducing_equivalent","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae04c12f-c0ac-5c8d-9ee2-76e58156d105","slug":"gsr","display_name":"Glutathione reductase / GSR","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ab091982-3acb-5a87-80fb-85dfe292c1e0","slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/ala-research/7632170.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"2ebbc851e5279e646bc7c892645c931a3fd2993830c3f4405379839cbbd6e707\", \"start_char\": 0, \"end_char\": 1863, \"text_sha256\": \"2ebbc851e5279e646bc7c892645c931a3fd2993830c3f4405379839cbbd6e707\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human erythrocytes, one G6PD-deficient donor and purified-enzyme assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Lipoic acid with glucose/2-deoxyglucose and G6PD inhibition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The deficient-donor finding is small and mechanistic; it is not a clinical safety or efficacy trial in G6PD deficiency.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lipoic-acid","display_name":"Lipoic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An enzyme associated with glutathione recycling can also reduce free lipoic acid.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ala-p7632170] Reduction and transport of lipoic acid by human erythrocytes. (1995). https://pubmed.ncbi.nlm.nih.gov/7632170/ DOI: 10.1016/0006-2952(95)00084-d","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Erythrocytes and glutathione reductase","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"32a5e9bc-170b-55df-b376-463f83c18bdb","evidence_kind":"source_excerpt","locator":"Lines 741-752","start_line":741,"end_line":752,"excerpt":"### ala-gsr-free-lipoate\nPurified human glutathione reductase reduced free lipoic acid to DHLA in vitro.\nCondition category: normal\nnutrient_topic: Alpha-lipoic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: An enzyme associated with glutathione recycling can also reduce free lipoic acid.\norganism: Human\ntissue_or_cell_type: Erythrocytes and glutathione reductase\nexperimental_model: Human erythrocytes, one G6PD-deficient donor and purified-enzyme assays\nlimitations: The deficient-donor finding is small and mechanistic; it is not a clinical safety or efficacy trial in G6PD deficiency.\nexposure: Lipoic acid with glucose/2-deoxyglucose and G6PD inhibition\nevidence_span: {\"source_cache\": \"artifacts/ala-research/7632170.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"2ebbc851e5279e646bc7c892645c931a3fd2993830c3f4405379839cbbd6e707\", \"start_char\": 0, \"end_char\": 1863, \"text_sha256\": \"2ebbc851e5279e646bc7c892645c931a3fd2993830c3f4405379839cbbd6e707\"}\n[ala-p7632170] Reduction and transport of lipoic acid by human erythrocytes. (1995). https://pubmed.ncbi.nlm.nih.gov/7632170/ DOI: 10.1016/0006-2952(95)00084-d","model_system":"Human erythrocytes, one G6PD-deficient donor and purified-enzyme assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ala-p7632170] Reduction and transport of lipoic acid by human erythrocytes. (1995). https://pubmed.ncbi.nlm.nih.gov/7632170/ DOI: 10.1016/0006-2952(95)00084-d","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d8afa8c2-ced9-5b28-90ca-2ac120ec7202","stable_key":"import-5d8e27d8-6a74-5560-827f-3f90908bbc34","title":"Alpha-lipoic acid: cofactor assembly, redox signaling and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"611da198ab85a272eb26b64ee330ef6d1a762853a8481f3191c4acfca9f3cf3d","revision_id":"618b4ce6-3157-5222-8325-408064be5ea5","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}