{"id":"e41fdca2-17a3-5670-892d-6d0fc72831e3","stable_key":"research:srxn-repair","predicate":"repairs_to","statement":"Sulfiredoxin catalyzes ATP-dependent repair of typical 2-Cys peroxiredoxin sulfinic acid.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"positive","is_public":true,"mechanism_event_id":"d05cef8b-19d5-5981-9b2b-1496aae100b3","mechanism_event_label":"Some overoxidized cysteine enzymes can be repaired.","subject":{"id":"d00ebaea-6ed6-5acd-b824-4827fa8418f8","slug":"srxn1","display_name":"SRXN1","entity_type_key":"protein"},"object":{"id":"64337aa7-dd2c-57da-af44-e71e0035cd9c","slug":"repaired-2cys-prx","display_name":"repaired typical 2-Cys peroxiredoxin","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"d05cef8b-19d5-5981-9b2b-1496aae100b3","stable_key":"research:srxn-repair","event_type":"redox_repair","label":"Some overoxidized cysteine enzymes can be repaired.","description":"Sulfiredoxin catalyzes ATP-dependent repair of typical 2-Cys peroxiredoxin sulfinic acid.","status":"active","compartment":null,"participants":[{"entity":{"id":"88626af4-09e1-568d-8bcb-25d13465baba","slug":"hyperoxidized-2cys-prx","display_name":"sulfinic-acid typical 2-Cys peroxiredoxin","entity_type_key":"protein_state"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d00ebaea-6ed6-5acd-b824-4827fa8418f8","slug":"srxn1","display_name":"SRXN1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"64337aa7-dd2c-57da-af44-e71e0035cd9c","slug":"repaired-2cys-prx","display_name":"repaired typical 2-Cys peroxiredoxin","entity_type_key":"protein_state"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"Purified enzymes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human sulfiredoxin/peroxiredoxin enzymology","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not establish repair of GPX4-Cys or every cysteine sulfinic acid.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9b703062-75e1-56c7-8ca8-091f2a989d78","evidence_kind":"curated_literature_summary","locator":"lines 1251-1261","start_line":1251,"end_line":1261,"excerpt":"## srxn-repair\n\nSome overoxidized cysteine enzymes can be repaired.\n\nSulfiredoxin catalyzes ATP-dependent repair of typical 2-Cys peroxiredoxin sulfinic acid.\n\nOrganism: Homo sapiens\nCell type: Purified enzymes\nExperimental model: Purified human sulfiredoxin/peroxiredoxin enzymology\nLimitations: Does not establish repair of GPX4-Cys or every cysteine sulfinic acid.\nPrimary reference: [Reduction of cysteine sulfinic acid in peroxiredoxin by sulfiredoxin proceeds directly through a sulfinic phosphoryl ester intermediate](https://pubmed.ncbi.nlm.nih.gov/18579529/)","model_system":"Purified human sulfiredoxin/peroxiredoxin enzymology","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[{"id":"8cbd2f31-0e86-5b3b-b0e2-9e1e58e012d2","title":"Some hyperoxidized cysteine enzymes can be repaired","kind":"qualification","status":"qualified","why":"Sulfiredoxin-mediated peroxiredoxin sulfinic-acid repair contradicts a blanket irreversibility claim.","resolution":"Keep GPX4-specific vulnerability separate from universal sulfur chemistry.","created_at":"2026-09-17 07:19:34","record_type":"qualification","display_label":"Source qualification","record_url":"/corrections/8cbd2f31-0e86-5b3b-b0e2-9e1e58e012d2","literature_review":{"revision_id":"80984e03-5f0f-5877-8094-afef7637444e","start_line":1577,"end_line":1582,"papers":[{"paper_key":"sulfiredoxin-2008","title":"Reduction of cysteine sulfinic acid in peroxiredoxin by sulfiredoxin proceeds directly through a sulfinic phosphoryl ester intermediate","url":"https://pubmed.ncbi.nlm.nih.gov/18579529/","doi":null,"year":2008,"model":"Purified human sulfiredoxin/peroxiredoxin enzymology","summary":"ATP-dependent repair is an exception to universal irreversible cysteine oxidation."}]},"sides":[{"conflict_id":"8cbd2f31-0e86-5b3b-b0e2-9e1e58e012d2","ordinal":0,"label":"Original preserved statement","revision_id":"1fa1ebde-141f-5d47-8a4f-ba87ff84a6c6","start_line":57,"end_line":59,"quote":"Cys-SH → Cys-SOH (sulfenic) → **Cys-SO₂H (sulfinic)** → Cys-SO₃H\n\nThat second arrow is essentially **one-way**. Overoxidize a catalytic cysteine and the enzyme is dead.","claim_id":null,"source_key":"molecular","source_title":"Selenium: the molecular cascade","claim_ids":[]},{"conflict_id":"8cbd2f31-0e86-5b3b-b0e2-9e1e58e012d2","ordinal":1,"label":"Original preserved statement","revision_id":"f8f97804-c1fb-56df-9c06-c02e36e2a5b8","start_line":313,"end_line":313,"quote":"Selenocysteine has unusual redox chemistry that can support rapid catalytic cycling. However, selenium's biological function should not be reduced to one slogan such as “a reversibility device”: selenoproteins also have roles in thyroid hormone metabolism, protein folding, membrane lipid protection, selenium transport, and other processes.","claim_id":null,"source_key":"deficiency","source_title":"Selenium deficiency: a mechanism-first reference","claim_ids":[]},{"conflict_id":"8cbd2f31-0e86-5b3b-b0e2-9e1e58e012d2","ordinal":2,"label":"Literature correction and experimental limits","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","start_line":1577,"end_line":1582,"quote":"## Some hyperoxidized cysteine enzymes can be repaired\n\nSulfiredoxin-mediated peroxiredoxin sulfinic-acid repair contradicts a blanket irreversibility claim.\n\nKeep GPX4-specific vulnerability separate from universal sulfur chemistry.\nPrimary reference: [Reduction of cysteine sulfinic acid in peroxiredoxin by sulfiredoxin proceeds directly through a sulfinic phosphoryl ester intermediate](https://pubmed.ncbi.nlm.nih.gov/18579529/)","claim_id":null,"source_key":"selenium-research-2026-09-17","source_title":"Selenium: literature corrections and mechanism additions","claim_ids":[]}]}],"research":{"topic":"Selenium scientific audit","plain_language":"Some overoxidized cysteine enzymes can be repaired.","evidence_scope":"direct_experimental","papers":[{"key":"sulfiredoxin-2008","title":"Reduction of cysteine sulfinic acid in peroxiredoxin by sulfiredoxin proceeds directly through a sulfinic phosphoryl ester intermediate","url":"https://pubmed.ncbi.nlm.nih.gov/18579529/","doi":null,"year":2008,"model":"Purified human sulfiredoxin/peroxiredoxin enzymology","summary":"ATP-dependent repair is an exception to universal irreversible cysteine oxidation."}]}}