{"id":"e41d2265-de68-5fb0-ba53-c6df18f45829","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-intestinal-ko-uptake","predicate":"reduces","statement":"Intestine-specific Slc52a3 deletion severely reduced carrier-mediated riboflavin uptake in mouse jejunal/colonic loops and isolated enterocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"41e299c5-ec24-5186-b055-971d3640e169","mechanism_event_label":"Loss of intestinal RFVT3 restricts riboflavin absorption.","subject":{"id":"a6411290-d3df-5372-baec-1ce8f0b7c952","slug":"mouse-intestinal-slc52a3-null","display_name":"Intestine-specific Slc52a3-null mouse genotype","entity_type_key":"gene"},"object":{"id":"8926ef8b-dbce-551f-9f14-160a318bb2eb","slug":"intestinal-riboflavin-uptake","display_name":"Intestinal riboflavin uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"41e299c5-ec24-5186-b055-971d3640e169","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-intestinal-ko-uptake-event","event_type":"biochemical_relationship","label":"Loss of intestinal RFVT3 restricts riboflavin absorption.","description":"Intestine-specific Slc52a3 deletion severely reduced carrier-mediated riboflavin uptake in mouse jejunal/colonic loops and isolated enterocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e120e61a-8a6c-5ed3-af13-e608616163ed","slug":"mouse-slc52a3","display_name":"Mouse riboflavin transporter 3 / Slc52a3","entity_type_key":"protein"},"role":"impaired-transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"86eb1eee-a8d1-539c-8c17-0911f69b6f1b","slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a6411290-d3df-5372-baec-1ce8f0b7c952","slug":"mouse-intestinal-slc52a3-null","display_name":"Intestine-specific Slc52a3-null mouse genotype","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8926ef8b-dbce-551f-9f14-160a318bb2eb","slug":"intestinal-riboflavin-uptake","display_name":"Intestinal riboflavin uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_location","value_text":"Effect of loss of RFVT-3 on intestinal RF uptake","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Conditional knockout with in vivo loops and isolated enterocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Intestinal Slc52a3 deletion versus littermate controls.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Residual uptake and species differences limit generalization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Loss of intestinal RFVT3 restricts riboflavin absorption.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[transport-intestinal-ko-2016] Conditional (intestinal-specific) knockout of the riboflavin transporter-3 (RFVT-3) impairs riboflavin absorption. (2016). https://pubmed.ncbi.nlm.nih.gov/26660539/ DOI: 10.1152/ajpgi.00340.2015","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Jejunum, colon and isolated intestinal epithelium","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"ebd7f846-ddff-53b7-bd6d-cb1ba3d76a69","evidence_kind":"source_excerpt","locator":"Lines 267-278","start_line":267,"end_line":278,"excerpt":"### transport-intestinal-ko-uptake\nIntestine-specific Slc52a3 deletion severely reduced carrier-mediated riboflavin uptake in mouse jejunal/colonic loops and isolated enterocytes.\nCondition category: machinery_impairment\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Loss of intestinal RFVT3 restricts riboflavin absorption.\norganism: Mus musculus\ntissue_or_cell_type: Jejunum, colon and isolated intestinal epithelium\nexperimental_model: Conditional knockout with in vivo loops and isolated enterocytes\nlimitations: Residual uptake and species differences limit generalization.\nexposure: Intestinal Slc52a3 deletion versus littermate controls.\nevidence_location: Effect of loss of RFVT-3 on intestinal RF uptake\n[transport-intestinal-ko-2016] Conditional (intestinal-specific) knockout of the riboflavin transporter-3 (RFVT-3) impairs riboflavin absorption. 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