{"id":"e3c7dd9c-35e0-55c4-b9f6-ce7c197889ec","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-glyt1-transport","predicate":"cotransports","statement":"Human GlyT1 expressed in COS-7 cells transported glycine with sodium and chloride; the kinetic analysis used the established 2 Na+:1 Cl-:1 glycine coupling.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2448f9a7-89e7-5960-a00a-8402eb588e7e","mechanism_event_label":"Glycine uptake draws on ion gradients, not just the amount of amino acid outside.","subject":{"id":"f9b4b084-aace-5bd4-a98b-f41923bf4923","slug":"slc6a9","display_name":"Human glycine transporter 1 / SLC6A9","entity_type_key":"protein"},"object":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"2448f9a7-89e7-5960-a00a-8402eb588e7e","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-glyt1-transport-event","event_type":"observed_relationship","label":"Glycine uptake draws on ion gradients, not just the amount of amino acid outside.","description":"Human GlyT1 expressed in COS-7 cells transported glycine with sodium and chloride; the kinetic analysis used the established 2 Na+:1 Cl-:1 glycine coupling.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f9b4b084-aace-5bd4-a98b-f41923bf4923","slug":"slc6a9","display_name":"Human glycine transporter 1 / SLC6A9","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6f35aa38-f875-5759-b749-97a2f6480ff3","slug":"chloride-ion","display_name":"Chloride ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human transporter in nonhuman COS-7 host cells; whole-cell electrophysiology, usually 1 mM glycine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The stoichiometry is established background incorporated into this primary kinetic study; no dietary salt intervention. Human protein and host-cell species differ.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Glycine uptake draws on ion gradients, not just the amount of amino acid outside.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A comparison of the transport kinetics of glycine transporter 1 and glycine transporter 2. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31270129/ · DOI 10.1085/jgp.201912318","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Sodium- and chloride-coupled glycine transport, which is inward.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Sodium- and chloride-coupled glycine transport, which is inward.","entity":null}],"evidence":[{"id":"7c9eb515-99a8-554d-bbbb-22f185407171","evidence_kind":"source_excerpt","locator":"Lines 26-32","start_line":26,"end_line":32,"excerpt":"## glycine-glyt1-transport\nGlycine uptake draws on ion gradients, not just the amount of amino acid outside.\nHuman GlyT1 expressed in COS-7 cells transported glycine with sodium and chloride; the kinetic analysis used the established 2 Na+:1 Cl-:1 glycine coupling.\nModel: Human transporter in nonhuman COS-7 host cells; whole-cell electrophysiology, usually 1 mM glycine.\nLimitations: The stoichiometry is established background incorporated into this primary kinetic study; no dietary salt intervention. Human protein and host-cell species differ.\nEvidence access: Primary full text\nA comparison of the transport kinetics of glycine transporter 1 and glycine transporter 2. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31270129/ · DOI 10.1085/jgp.201912318","model_system":"Human transporter in nonhuman COS-7 host cells; whole-cell electrophysiology, usually 1 mM glycine.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"4fabd2fc8e71ca156c91023e7340be3f5a02ae03e91d3a24b231c2adfb0de0aa","revision_id":"24801e49-34a8-53c3-bdf5-b59143441450","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}