{"id":"e39a9448-6fdc-512d-bf0a-05a879b39263","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-flad1-isoform1-mitochondria","predicate":"localizes-to","statement":"In vitro synthesized human FADS1 entered isolated rat liver mitochondria and was processed; transfected-cell imaging also localized it to mitochondria.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"c0a8c561-35b7-5b17-96c7-4aa0bf35f83e","mechanism_event_label":"One FLAD1 isoform can provide FAD-synthesis machinery inside mitochondria.","subject":{"id":"b872ce95-e881-5fcb-be38-8d8f8b0156e8","slug":"flad1-isoform-1","display_name":"Human FAD synthetase isoform 1","entity_type_key":"protein"},"object":{"id":"f5bd4edd-4298-5985-976f-897fd415c3d5","slug":"mitochondria","display_name":"Mitochondria","entity_type_key":"organelle"},"evidence_count":1,"mechanism_event":{"id":"c0a8c561-35b7-5b17-96c7-4aa0bf35f83e","stable_key":"548ab9d6-3a9b-5bed-879c-17d03813b636:transport-flad1-isoform1-mitochondria-event","event_type":"biochemical_relationship","label":"One FLAD1 isoform can provide FAD-synthesis machinery inside mitochondria.","description":"In vitro synthesized human FADS1 entered isolated rat liver mitochondria and was processed; transfected-cell imaging also localized it to mitochondria.","status":"provisional","compartment":{"slug":"mitochondria","display_name":"Mitochondria"},"participants":[{"entity":{"id":"b872ce95-e881-5fcb-be38-8d8f8b0156e8","slug":"flad1-isoform-1","display_name":"Human FAD synthetase isoform 1","entity_type_key":"protein"},"role":"imported-protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"f5bd4edd-4298-5985-976f-897fd415c3d5","slug":"mitochondria","display_name":"Mitochondria","entity_type_key":"organelle"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_location","value_text":"Abstract; mitochondrial import and confocal imaging","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Import into purified rat liver mitochondria; BHK-21/Caco-2 confocal imaging","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"In vitro synthesized and transiently expressed human FADS1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Construct/import evidence does not establish its abundance in every human tissue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Riboflavin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"riboflavin","display_name":"Riboflavin (vitamin B2)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human protein; rat mitochondria; hamster and human cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"One FLAD1 isoform can provide FAD-synthesis machinery inside mitochondria.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[transport-fads-localization-2010] Mitochondrial localization of human FAD synthetase isoform 1. (2010). https://pubmed.ncbi.nlm.nih.gov/20060505/ DOI: 10.1016/j.mito.2009.12.149","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Liver mitochondria and cultured epithelial cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f28f3d8c-9874-5c4d-93a5-632f4be2f303","evidence_kind":"source_excerpt","locator":"Lines 347-358","start_line":347,"end_line":358,"excerpt":"### transport-flad1-isoform1-mitochondria\nIn vitro synthesized human FADS1 entered isolated rat liver mitochondria and was processed; transfected-cell imaging also localized it to mitochondria.\nCondition category: normal\nnutrient_topic: Riboflavin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: One FLAD1 isoform can provide FAD-synthesis machinery inside mitochondria.\norganism: Human protein; rat mitochondria; hamster and human cells\ntissue_or_cell_type: Liver mitochondria and cultured epithelial cells\nexperimental_model: Import into purified rat liver mitochondria; BHK-21/Caco-2 confocal imaging\nlimitations: Construct/import evidence does not establish its abundance in every human tissue.\nexposure: In vitro synthesized and transiently expressed human FADS1.\nevidence_location: Abstract; mitochondrial import and confocal imaging\n[transport-fads-localization-2010] Mitochondrial localization of human FAD synthetase isoform 1. (2010). https://pubmed.ncbi.nlm.nih.gov/20060505/ DOI: 10.1016/j.mito.2009.12.149","model_system":"Import into purified rat liver mitochondria; BHK-21/Caco-2 confocal imaging","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [transport-fads-localization-2010] Mitochondrial localization of human FAD synthetase isoform 1. (2010). https://pubmed.ncbi.nlm.nih.gov/20060505/ DOI: 10.1016/j.mito.2009.12.149","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f7c9578-82bf-5e2d-b5c4-72a79fb4f6af","stable_key":"import-548ab9d6-3a9b-5bed-879c-17d03813b636","title":"Riboflavin: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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