{"id":"e3973f7a-e36b-5721-b839-272cce5b9bbe","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-ip3-decrease","predicate":"decreases","statement":"Lithium lowered IP3 in the COS-7 experiments; added myo-inositol raised it relative to lithium alone.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"863df73f-9c53-5570-955d-1fcfef32e672","mechanism_event_label":"Availability of the precursor changes a signaling messenger.","subject":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"object":{"id":"7005bbc2-d4b5-5903-b776-94dd2288d35f","slug":"ip3","display_name":"IP3","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"863df73f-9c53-5570-955d-1fcfef32e672","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-ip3-decrease-event","event_type":"observed_relationship","label":"Availability of the precursor changes a signaling messenger.","description":"Lithium lowered IP3 in the COS-7 experiments; added myo-inositol raised it relative to lithium alone.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7005bbc2-d4b5-5903-b776-94dd2288d35f","slug":"ip3","display_name":"IP3","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e2e3902-5e26-52e3-9c85-1d7c0ace1637","slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"50afe95c-f542-5511-ba8b-c2ff2b037977","slug":"myo-inositol","display_name":"Myo-inositol","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"96843812-ebe3-5ff9-977d-97341dfb7eba","slug":"monkey-cos7-inositol-monophosphatase","display_name":"Monkey COS-7-cell inositol monophosphatase activity","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Monkey COS-7; 10 mM LiCl, 1 mM myo-inositol rescue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-culture rescue is not a clinical supplement recommendation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lithium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"Availability of the precursor changes a signaling messenger.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Lithium induces autophagy by inhibiting inositol monophosphatase. · 2005 · https://pubmed.ncbi.nlm.nih.gov/16186256/ · DOI 10.1083/jcb.200504035","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"856e03dd-b285-5c0b-bc51-12c76fcbf110","evidence_kind":"source_excerpt","locator":"Lines 80-86","start_line":80,"end_line":86,"excerpt":"## lithium-ip3-decrease\nAvailability of the precursor changes a signaling messenger.\nLithium lowered IP3 in the COS-7 experiments; added myo-inositol raised it relative to lithium alone.\nModel: Monkey COS-7; 10 mM LiCl, 1 mM myo-inositol rescue.\nLimitations: Cell-culture rescue is not a clinical supplement recommendation.\nEvidence access: Primary full text\nLithium induces autophagy by inhibiting inositol monophosphatase. · 2005 · https://pubmed.ncbi.nlm.nih.gov/16186256/ · DOI 10.1083/jcb.200504035","model_system":"Monkey COS-7; 10 mM LiCl, 1 mM myo-inositol rescue.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b2dd2f3c-5c4c-5a12-9a0d-91b521ffcf98","stable_key":"import-b7798a90-72a3-5454-a321-c4be88bf0cc4","title":"Lithium: metal-sensitive enzymes, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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