{"id":"e365a115-f17f-542f-9a9c-3fa4e88201b0","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-liver-cyp1a2","predicate":"induces_in_liver_slices","statement":"DIM increased CYP1A2 protein in cultured human liver slices.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9fdfbf78-525a-5e29-ad6c-8f2943cf2406","mechanism_event_label":"Human liver tissue can make more of this enzyme after DIM exposure.","subject":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"object":{"id":"9b15c13c-a369-5d10-a76a-525e8b19221d","slug":"cyp1a2","display_name":"Human cytochrome P450 1A2","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"9fdfbf78-525a-5e29-ad6c-8f2943cf2406","stable_key":"182336c6-ed36-5ec6-8a09-25c31096262e:dim-liver-cyp1a2-event","event_type":"biochemical_relationship","label":"Human liver tissue can make more of this enzyme after DIM exposure.","description":"DIM increased CYP1A2 protein in cultured human liver slices.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6009bdd4-f1b5-5867-b5c3-cdf7c9645cd0","slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9b15c13c-a369-5d10-a76a-525e8b19221d","slug":"cyp1a2","display_name":"Human cytochrome P450 1A2","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/dim-research/9741959.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"11eec60fad1ec0e18085a59f90fa3237235bb0614b5ee2b29058757e3b45ba47\", \"start_char\": 0, \"end_char\": 1338, \"text_sha256\": \"11eec60fad1ec0e18085a59f90fa3237235bb0614b5ee2b29058757e3b45ba47\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Precision-cut liver-slice induction and immunoblotting","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"DIM 50 micromolar for 72 hours","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ex-vivo exposure; not a measured oral DIM effect on a patient drug concentration. Four donors showed variable responses.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"dim","display_name":"3,3'-Diindolylmethane / DIM","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human donor liver slices","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Human liver tissue can make more of this enzyme after DIM exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dim-p9741959] 3,3'-Diindolylmethane induces CYP1A2 in cultured precision-cut human liver slices. (1998). https://pubmed.ncbi.nlm.nih.gov/9741959/ DOI: 10.1080/004982598239227","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"CYP1A proteins and catalytic assays","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"14ecfcc8-02c1-5c3b-b507-766fed6dbd9c","evidence_kind":"source_excerpt","locator":"Lines 350-361","start_line":350,"end_line":361,"excerpt":"### dim-liver-cyp1a2\nDIM increased CYP1A2 protein in cultured human liver slices.\nCondition category: normal\nnutrient_topic: Diindolylmethane (DIM) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Human liver tissue can make more of this enzyme after DIM exposure.\norganism: Human donor liver slices\ntissue_or_cell_type: CYP1A proteins and catalytic assays\nexperimental_model: Precision-cut liver-slice induction and immunoblotting\nlimitations: Ex-vivo exposure; not a measured oral DIM effect on a patient drug concentration. Four donors showed variable responses.\nexposure: DIM 50 micromolar for 72 hours\nevidence_span: {\"source_cache\": \"artifacts/dim-research/9741959.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"11eec60fad1ec0e18085a59f90fa3237235bb0614b5ee2b29058757e3b45ba47\", \"start_char\": 0, \"end_char\": 1338, \"text_sha256\": \"11eec60fad1ec0e18085a59f90fa3237235bb0614b5ee2b29058757e3b45ba47\"}\n[dim-p9741959] 3,3'-Diindolylmethane induces CYP1A2 in cultured precision-cut human liver slices. (1998). https://pubmed.ncbi.nlm.nih.gov/9741959/ DOI: 10.1080/004982598239227","model_system":"Precision-cut liver-slice induction and immunoblotting","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [dim-p9741959] 3,3'-Diindolylmethane induces CYP1A2 in cultured precision-cut human liver slices. 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