{"id":"e3252fbf-625e-5622-9603-73c6e83423a7","stable_key":"fe6af7fc-7372-5c4f-9c2f-2bbf56695397:phosphorus-nherf-napi","predicate":"fails_to_induce","statement":"PTH-(3–34), used to stimulate apical receptors, failed to induce NaPi-IIa internalization in Nherf1-deficient mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"c62c0e6d-68a3-50e7-8a9d-58625d41e6cc","mechanism_event_label":"The isolated apical route failed, unlike the broader PTH-(1–34) stimulation.","subject":{"id":"7e108942-bdad-5c52-a067-efbb0b8bfb72","slug":"pth-3-34","display_name":"PTH-(3–34) experimental peptide","entity_type_key":"small_molecule"},"object":{"id":"2b02245d-258e-5ebf-b751-940fc3d330f8","slug":"mouse-napi2a-internalization","display_name":"Mouse renal NaPi-IIa internalization from the brush border","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"c62c0e6d-68a3-50e7-8a9d-58625d41e6cc","stable_key":"fe6af7fc-7372-5c4f-9c2f-2bbf56695397:phosphorus-nherf-napi-event","event_type":"biochemical_relationship","label":"The isolated apical route failed, unlike the broader PTH-(1–34) stimulation.","description":"PTH-(3–34), used to stimulate apical receptors, failed to induce NaPi-IIa internalization in Nherf1-deficient mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a6756504-724e-50c7-a853-11ac7b330527","slug":"mouse-nherf1-null","display_name":"Experimental mouse Nherf1 knockout","entity_type_key":"protein_state"},"role":"genotype","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c302c2c9-af8b-5480-a8b9-c46379f420b2","slug":"mouse-slc34a1","display_name":"Mouse sodium-phosphate cotransporter NaPi-IIa / Slc34a1","entity_type_key":"protein"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7e108942-bdad-5c52-a067-efbb0b8bfb72","slug":"pth-3-34","display_name":"PTH-(3–34) experimental peptide","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2b02245d-258e-5ebf-b751-940fc3d330f8","slug":"mouse-napi2a-internalization","display_name":"Mouse renal NaPi-IIa internalization from the brush border","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/phosphorus-research/16987995.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"c5a055252ffc61da98e24d1de3a1733ee7aea678aded8cbdf071d18ad707d0bc\", \"start_char\": 0, \"end_char\": 1699, \"text_sha256\": \"c5a055252ffc61da98e24d1de3a1733ee7aea678aded8cbdf071d18ad707d0bc\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Receptor-fragment stimulation, knockout and signaling-bypass experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"PTH-(1–34), apically selective PTH-(3–34), PKC/PKA pathway stimulation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Apical and basolateral receptor routes differ. Loss of one scaffold does not eliminate every PTH response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Phosphorus research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"phosphorus","display_name":"Phosphorus","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The isolated apical route failed, unlike the broader PTH-(1–34) stimulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[phosphorus-p16987995] Defective coupling of apical PTH receptors to phospholipase C prevents internalization of the Na+-phosphate cotransporter NaPi-IIa in Nherf1-deficient mice. (2007). https://pubmed.ncbi.nlm.nih.gov/16987995/ DOI: 10.1152/ajpcell.00126.2006","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Renal proximal tubule brush-border membrane","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"5ce8d6ec-7f90-5951-ad18-510f72bf488f","evidence_kind":"source_excerpt","locator":"Lines 425-436","start_line":425,"end_line":436,"excerpt":"### phosphorus-nherf-napi\nPTH-(3–34), used to stimulate apical receptors, failed to induce NaPi-IIa internalization in Nherf1-deficient mice.\nCondition category: machinery_impairment\nnutrient_topic: Phosphorus research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The isolated apical route failed, unlike the broader PTH-(1–34) stimulation.\norganism: Mouse\ntissue_or_cell_type: Renal proximal tubule brush-border membrane\nexperimental_model: Receptor-fragment stimulation, knockout and signaling-bypass experiments\nlimitations: Apical and basolateral receptor routes differ. Loss of one scaffold does not eliminate every PTH response.\nexposure: PTH-(1–34), apically selective PTH-(3–34), PKC/PKA pathway stimulation\nevidence_span: {\"source_cache\": \"artifacts/phosphorus-research/16987995.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"c5a055252ffc61da98e24d1de3a1733ee7aea678aded8cbdf071d18ad707d0bc\", \"start_char\": 0, \"end_char\": 1699, \"text_sha256\": \"c5a055252ffc61da98e24d1de3a1733ee7aea678aded8cbdf071d18ad707d0bc\"}\n[phosphorus-p16987995] Defective coupling of apical PTH receptors to phospholipase C prevents internalization of the Na+-phosphate cotransporter NaPi-IIa in Nherf1-deficient mice. (2007). https://pubmed.ncbi.nlm.nih.gov/16987995/ DOI: 10.1152/ajpcell.00126.2006","model_system":"Receptor-fragment stimulation, knockout and signaling-bypass experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [phosphorus-p16987995] Defective coupling of apical PTH receptors to phospholipase C prevents internalization of the Na+-phosphate cotransporter NaPi-IIa in Nherf1-deficient mice. (2007). https://pubmed.ncbi.nlm.nih.gov/16987995/ DOI: 10.1152/ajpcell.00126.2006","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"cfdefe70-3a09-5e2e-97c8-46626382e4b4","stable_key":"import-fe6af7fc-7372-5c4f-9c2f-2bbf56695397","title":"Phosphorus: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a04956d7177582b749608db404e6b691ae3b28a9f2f762781b6d820b461044fb","revision_id":"713b78f5-1133-5b2b-8822-20c0852e3715","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}