{"id":"e2f9cc7c-b7eb-5424-afd4-8d8020b66c04","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-pregnancy-3hia","predicate":"has_context_dependent_marker","statement":"At the controlled intake, pregnant women excreted 69% more 3-HIA than controls, while urinary 3-HIA-carnitine was 27% lower.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0b2a9268-3986-5f10-b78d-a1decf31b2cc","mechanism_event_label":"Two related markers moved in opposite directions during pregnancy.","subject":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"object":{"id":"ebbcb656-4274-5f50-a1be-9d69df5e3434","slug":"human-urinary-3hia","display_name":"Human urinary 3-hydroxyisovaleric acid excretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0b2a9268-3986-5f10-b78d-a1decf31b2cc","stable_key":"08ce9896-9d1c-5bbf-b705-5bfe771091d5:b7-pregnancy-3hia-event","event_type":"observed_intervention","label":"Two related markers moved in opposite directions during pregnancy.","description":"At the controlled intake, pregnant women excreted 69% more 3-HIA than controls, while urinary 3-HIA-carnitine was 27% lower.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a316342b-d919-5369-a101-40392590658a","slug":"3-hydroxyisovaleric-acid","display_name":"3-Hydroxyisovaleric acid / 3-HIA","entity_type_key":"small_molecule"},"role":"marker","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8f222f3c-c671-52e4-bde5-1093e56ad486","slug":"3-hydroxyisovalerylcarnitine","display_name":"3-Hydroxyisovalerylcarnitine / 3-HIA-carnitine","entity_type_key":"small_molecule"},"role":"second marker","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"possible modifying factor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"37a8e96b-f95b-5ba7-a0bc-8ed3cfaf5fd8","slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ebbcb656-4274-5f50-a1be-9d69df5e3434","slug":"human-urinary-3hia","display_name":"Human urinary 3-hydroxyisovaleric acid excretion","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/biotin-research/25122647.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"6066b007a013c3c0c69336e7aba0e51baa39e3a24b6eb918cb4778e792376a9b\", \"start_char\": 0, \"end_char\": 2354, \"text_sha256\": \"6066b007a013c3c0c69336e7aba0e51baa39e3a24b6eb918cb4778e792376a9b\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Controlled feeding: 26 pregnant, 28 lactating and 21 control women","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"57 micrograms dietary biotin per day for 10–12 weeks","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Groups differed in reproductive state. Marker changes do not establish fetal outcomes, universal deficiency or a treatment dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Biotin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"biotin","display_name":"Biotin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Two related markers moved in opposite directions during pregnancy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b7-p25122647] Pregnancy and lactation alter biomarkers of biotin metabolism in women consuming a controlled diet. (2014). https://pubmed.ncbi.nlm.nih.gov/25122647/ DOI: 10.3945/jn.114.194472","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Urine and plasma","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"56a9eee9-69cb-5044-8df2-16c88c9f5003","evidence_kind":"source_excerpt","locator":"Lines 988-999","start_line":988,"end_line":999,"excerpt":"### b7-pregnancy-3hia\nAt the controlled intake, pregnant women excreted 69% more 3-HIA than controls, while urinary 3-HIA-carnitine was 27% lower.\nCondition category: biomarker_context\nnutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Two related markers moved in opposite directions during pregnancy.\norganism: Homo sapiens\ntissue_or_cell_type: Urine and plasma\nexperimental_model: Controlled feeding: 26 pregnant, 28 lactating and 21 control women\nlimitations: Groups differed in reproductive state. Marker changes do not establish fetal outcomes, universal deficiency or a treatment dose.\nexposure: 57 micrograms dietary biotin per day for 10–12 weeks\nevidence_span: {\"source_cache\": \"artifacts/biotin-research/25122647.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"6066b007a013c3c0c69336e7aba0e51baa39e3a24b6eb918cb4778e792376a9b\", \"start_char\": 0, \"end_char\": 2354, \"text_sha256\": \"6066b007a013c3c0c69336e7aba0e51baa39e3a24b6eb918cb4778e792376a9b\"}\n[b7-p25122647] Pregnancy and lactation alter biomarkers of biotin metabolism in women consuming a controlled diet. (2014). https://pubmed.ncbi.nlm.nih.gov/25122647/ DOI: 10.3945/jn.114.194472","model_system":"Controlled feeding: 26 pregnant, 28 lactating and 21 control women","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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