{"id":"e2a1c50e-0f7a-5bd2-b7ec-19e93b38e253","stable_key":"44737fa3-b335-53f4-a644-b9878d4416ac:creatine-feedback-agat-expression","predicate":"reduced","statement":"Renal AGAT synthesis fell to 21% of control and functional AGAT mRNA to 37% with creatine feeding.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"352202fb-610f-569d-a527-42d9c91ecfa1","mechanism_event_label":"The body adjusted production of the synthesis enzyme, not merely the amount of precursor in a test tube.","subject":{"id":"9f5df558-ed3d-5cda-b5ca-0f90f7aa6a8c","slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"},"object":{"id":"aa8214b4-1fa1-5e0e-a1e2-5d35a69fad7e","slug":"agat-expression","display_name":"AGAT expression and synthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"352202fb-610f-569d-a527-42d9c91ecfa1","stable_key":"44737fa3-b335-53f4-a644-b9878d4416ac:creatine-feedback-agat-expression-event","event_type":"biochemical_relationship","label":"The body adjusted production of the synthesis enzyme, not merely the amount of precursor in a test tube.","description":"Renal AGAT synthesis fell to 21% of control and functional AGAT mRNA to 37% with creatine feeding.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8ad2e77e-c338-5a58-9c35-9a2a04031478","slug":"rat-gatm","display_name":"Rat glycine amidinotransferase / Gatm","entity_type_key":"protein"},"role":"regulated_enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9f5df558-ed3d-5cda-b5ca-0f90f7aa6a8c","slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"aa8214b4-1fa1-5e0e-a1e2-5d35a69fad7e","slug":"agat-expression","display_name":"AGAT expression and synthesis","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/creatine-research/6384218.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"12e742efaf02e310dd4a64db3d9336c23354b40d901631e61be2a0cbb9fee507\", \"start_char\": 0, \"end_char\": 1077, \"text_sha256\": \"12e742efaf02e310dd4a64db3d9336c23354b40d901631e61be2a0cbb9fee507\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Controlled feeding with enzyme, protein-synthesis and functional-mRNA measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Diet supplemented with 0.3% creatine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rat feedback regulation; percentages are relative to controls in this experiment, not human intake targets.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Creatine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"creatine","display_name":"Creatine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rats","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The body adjusted production of the synthesis enzyme, not merely the amount of precursor in a test tube.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[creatine-p6384218] Repression of rat kidney L-arginine:glycine amidinotransferase synthesis by creatine at a pretranslational level. (1984). https://pubmed.ncbi.nlm.nih.gov/6384218/ DOI: 10.1016/s0021-9258(20)71316-5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Kidney","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1361ab89-b769-593a-95bb-441e59f8af58","evidence_kind":"source_excerpt","locator":"Lines 243-254","start_line":243,"end_line":254,"excerpt":"### creatine-feedback-agat-expression\nRenal AGAT synthesis fell to 21% of control and functional AGAT mRNA to 37% with creatine feeding.\nCondition category: normal\nnutrient_topic: Creatine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The body adjusted production of the synthesis enzyme, not merely the amount of precursor in a test tube.\norganism: Rats\ntissue_or_cell_type: Kidney\nexperimental_model: Controlled feeding with enzyme, protein-synthesis and functional-mRNA measurements\nlimitations: Rat feedback regulation; percentages are relative to controls in this experiment, not human intake targets.\nexposure: Diet supplemented with 0.3% creatine\nevidence_span: {\"source_cache\": \"artifacts/creatine-research/6384218.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"12e742efaf02e310dd4a64db3d9336c23354b40d901631e61be2a0cbb9fee507\", \"start_char\": 0, \"end_char\": 1077, \"text_sha256\": \"12e742efaf02e310dd4a64db3d9336c23354b40d901631e61be2a0cbb9fee507\"}\n[creatine-p6384218] Repression of rat kidney L-arginine:glycine amidinotransferase synthesis by creatine at a pretranslational level. (1984). https://pubmed.ncbi.nlm.nih.gov/6384218/ DOI: 10.1016/s0021-9258(20)71316-5","model_system":"Controlled feeding with enzyme, protein-synthesis and functional-mRNA measurements","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [creatine-p6384218] Repression of rat kidney L-arginine:glycine amidinotransferase synthesis by creatine at a pretranslational level. (1984). https://pubmed.ncbi.nlm.nih.gov/6384218/ DOI: 10.1016/s0021-9258(20)71316-5","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"70358046-c5c3-5e9a-9fc9-eb01c3fff88b","stable_key":"import-44737fa3-b335-53f4-a644-b9878d4416ac","title":"Creatine: synthesis, transport, phosphocreatine energetics and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"548237d3e98a73a7dc02181807921fdd8b902adcee46248792258a761593eda9","revision_id":"88738255-8028-52fb-9c12-0fe92d8ce367","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}