{"id":"e250e9ef-eefb-5e0f-a95e-632586178902","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-amination-chemistry","predicate":"reacts_with_ammonia_to_form","statement":"Oxidized myricetin reacted chemically with ammonia to form 4′-NH2-myricetin, structurally confirmed by NMR and LC-MS.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fa2c967c-2bb6-574f-9f05-3dfda4b4d608","mechanism_event_label":"Reactive nitrogen can become part of a new metabolite.","subject":{"id":"834080ae-876c-5481-b4a3-d6bc8ad9fe95","slug":"myricetin-quinone","display_name":"Myricetin quinone, oxidation intermediate","entity_type_key":"small_molecule"},"object":{"id":"fa285e11-27d1-5736-9808-7c4be0767e83","slug":"4-prime-amino-myricetin","display_name":"4′-NH2-myricetin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"fa2c967c-2bb6-574f-9f05-3dfda4b4d608","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-amination-chemistry-event","event_type":"observed_relationship","label":"Reactive nitrogen can become part of a new metabolite.","description":"Oxidized myricetin reacted chemically with ammonia to form 4′-NH2-myricetin, structurally confirmed by NMR and LC-MS.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"834080ae-876c-5481-b4a3-d6bc8ad9fe95","slug":"myricetin-quinone","display_name":"Myricetin quinone, oxidation intermediate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fa285e11-27d1-5736-9808-7c4be0767e83","slug":"4-prime-amino-myricetin","display_name":"4′-NH2-myricetin","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1374ec24-1a5b-552b-982f-51f9082f4187","slug":"ammonia","display_name":"Ammonia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free chemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not an established ammonia-detoxification therapy or enzyme-catalyzed pathway.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Reactive nitrogen can become part of a new metabolite.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Biotransformation of Myricetin: A Novel Metabolic Pathway to Produce Aminated Products in Mice. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31087612/ · DOI 10.1002/mnfr.201900203","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"1112b74d-d936-5e0f-971a-3622d791bc16","evidence_kind":"source_excerpt","locator":"Lines 44-50","start_line":44,"end_line":50,"excerpt":"## myricetin-amination-chemistry\nReactive nitrogen can become part of a new metabolite.\nOxidized myricetin reacted chemically with ammonia to form 4′-NH2-myricetin, structurally confirmed by NMR and LC-MS.\nModel: Cell-free chemistry.\nLimitations: Not an established ammonia-detoxification therapy or enzyme-catalyzed pathway.\nEvidence access: Primary abstract\nBiotransformation of Myricetin: A Novel Metabolic Pathway to Produce Aminated Products in Mice. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31087612/ · DOI 10.1002/mnfr.201900203","model_system":"Cell-free chemistry.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0abf512275cf3aae9e4ec8544f1cde97e0155d0cfb5be12a193cdd5a898386a7","revision_id":"422e342f-a55c-5e73-ba98-43df560f49b9","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}