{"id":"e23fc7ee-19b1-519e-bd12-2492737e68ff","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-u266-ros","predicate":"increases","statement":"Fisetin increased ROS and AMPK-associated signaling in human U266 myeloma cells undergoing apoptosis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"4839fe52-8a8a-5582-91ed-e0b8edbfe82f","mechanism_event_label":"A pro-oxidant response can contribute to cell killing.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"12fc65dc-9d24-582e-a2bb-57f5f7c7cb34","slug":"human-u266-ros-generation","display_name":"Reactive-oxygen generation in human U266 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"4839fe52-8a8a-5582-91ed-e0b8edbfe82f","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-u266-ros-event","event_type":"observed_relationship","label":"A pro-oxidant response can contribute to cell killing.","description":"Fisetin increased ROS and AMPK-associated signaling in human U266 myeloma cells undergoing apoptosis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"12fc65dc-9d24-582e-a2bb-57f5f7c7cb34","slug":"human-u266-ros-generation","display_name":"Reactive-oxygen generation in human U266 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"95bc303f-ad04-59ea-8e1e-32f9e8b72c56","slug":"human-ampk-complexes","display_name":"Human AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human tumor cell culture.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different from protection of nonmalignant cells; not an established cancer therapy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A pro-oxidant response can contribute to cell killing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Activation of reactive oxygen species/AMP activated protein kinase signaling mediates fisetin-induced apoptosis in multiple myeloma U266 cells. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22261340/ · DOI 10.1016/j.canlet.2012.01.008","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"9eaf3431-f5c1-5e4b-9f07-a00609d423ff","evidence_kind":"source_excerpt","locator":"Lines 368-374","start_line":368,"end_line":374,"excerpt":"## fisetin-u266-ros\nA pro-oxidant response can contribute to cell killing.\nFisetin increased ROS and AMPK-associated signaling in human U266 myeloma cells undergoing apoptosis.\nModel: Human tumor cell culture.\nLimitations: Different from protection of nonmalignant cells; not an established cancer therapy.\nEvidence access: Primary abstract\nActivation of reactive oxygen species/AMP activated protein kinase signaling mediates fisetin-induced apoptosis in multiple myeloma U266 cells. · 2012 · https://pubmed.ncbi.nlm.nih.gov/22261340/ · DOI 10.1016/j.canlet.2012.01.008","model_system":"Human tumor cell culture.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}