{"id":"e1eafb0e-2da4-52e5-ad2d-0111fce191e2","stable_key":"05a3cf47-e660-5ab3-8c32-5eba9847cf0a:thc-cb2-cloned","predicate":"binds","statement":"The murine CB2 peripheral cannabinoid receptor was cloned, expressed and shown to function as a cannabinoid receptor distinct from the brain receptor.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"55d5c33e-24c7-54f5-aede-3ad1da4dfd2c","mechanism_event_label":"A second receptor exists, and it sits mostly outside the brain.","subject":{"id":"271db259-ea1a-5944-b510-d3aadef0a9d4","slug":"mouse-cnr2","display_name":"Mouse peripheral cannabinoid receptor CB2 / Cnr2","entity_type_key":"protein"},"object":{"id":"5a9e5b75-e6aa-5684-ad62-8a8d60148c84","slug":"tetrahydrocannabinol","display_name":"Delta-9-tetrahydrocannabinol / THC","entity_type_key":"drug"},"evidence_count":1,"mechanism_event":{"id":"55d5c33e-24c7-54f5-aede-3ad1da4dfd2c","stable_key":"05a3cf47-e660-5ab3-8c32-5eba9847cf0a:thc-cb2-cloned-event","event_type":"biochemical_relationship","label":"A second receptor exists, and it sits mostly outside the brain.","description":"The murine CB2 peripheral cannabinoid receptor was cloned, expressed and shown to function as a cannabinoid receptor distinct from the brain receptor.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5abfafd4-17c3-587a-8f02-e38cce8f8028","slug":"cnr2","display_name":"Human cannabinoid receptor 2 / CB2 / CNR2","entity_type_key":"protein"},"role":"human_orthologue","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"271db259-ea1a-5944-b510-d3aadef0a9d4","slug":"mouse-cnr2","display_name":"Mouse peripheral cannabinoid receptor CB2 / Cnr2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5a9e5b75-e6aa-5684-ad62-8a8d60148c84","slug":"tetrahydrocannabinol","display_name":"Delta-9-tetrahydrocannabinol / THC","entity_type_key":"drug"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/thc-research/8679694.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"6140a784856b6848c64e4be175012e45a0c7343fa524308753bee15fc57162d4\", \"start_char\": 0, \"end_char\": 967, \"text_sha256\": \"6140a784856b6848c64e4be175012e45a0c7343fa524308753bee15fc57162d4\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cloning, expression and functional characterisation of the murine CB2 receptor","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Heterologous expression with ligand binding","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Establishes a second, peripheral receptor. 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It is the mouse orthologue.\nexposure: Heterologous expression with ligand binding\nevidence_span: {\"source_cache\": \"artifacts/thc-research/8679694.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"6140a784856b6848c64e4be175012e45a0c7343fa524308753bee15fc57162d4\", \"start_char\": 0, \"end_char\": 967, \"text_sha256\": \"6140a784856b6848c64e4be175012e45a0c7343fa524308753bee15fc57162d4\"}\n[thc-p8679694] Molecular cloning, expression and function of the murine CB2 peripheral cannabinoid receptor. (1996). https://pubmed.ncbi.nlm.nih.gov/8679694/ DOI: 10.1016/0167-4781(96)00047-4","model_system":"Cloning, expression and functional characterisation of the murine CB2 receptor","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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